Detailed information of TA system TA092338

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4111781..4112383
• Replicon: chromosome
• Accession: NZ_CP025892
• Organism: Escherichia coli strain 503025

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CXX03_RS20455
• Protein ID: WP_000897305.1
• Coordinates: 4111781..4112092 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXX03_RS20460
• Protein ID: WP_000356397.1
• Coordinates: 4112093..4112383 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX03_RS20425	4106811..4107596	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
CXX03_RS20430	4107695..4108294	+	600	WP_001295269.1	glucose-1-phosphatase	-
CXX03_RS20435	4108288..4109160	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CXX03_RS20440	4109157..4109594	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CXX03_RS20445	4109639..4110580	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CXX03_RS20450	4110644..4111552	-	909	WP_001162704.1	alpha/beta hydrolase	-
CXX03_RS20455	4111781..4112092	+	312	WP_000897305.1	hypothetical protein	Toxin
CXX03_RS20460	4112093..4112383	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CXX03_RS20465	4112988..4113206	+	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
CXX03_RS20470	4113425..4113667	+	243	WP_001086388.1	hypothetical protein	-
CXX03_RS20475	4113997..4114926	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CXX03_RS20480	4114923..4115558	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CXX03_RS20485	4115555..4116457	-	903	WP_001717071.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T92338 WP_000897305.1 NZ_CP025892:4111781-4112092 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T92338 NZ_CP025892:4111781-4112092 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT92338 WP_000356397.1 NZ_CP025892:4112093-4112383 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT92338 NZ_CP025892:4112093-4112383 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7