Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2083178..2083680 Replicon chromosome
Accession NZ_CP025892
Organism Escherichia coli strain 503025

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXX03_RS10515 Protein ID WP_000767829.1
Coordinates 2083178..2083432 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXX03_RS10520 Protein ID WP_001259255.1
Coordinates 2083429..2083680 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX03_RS10485 2078194..2078421 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CXX03_RS10490 2078435..2079492 - 1058 Protein_2011 transport protein YeeE -
CXX03_RS10495 2079671..2081029 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXX03_RS10500 2081019..2081081 - 63 WP_010723108.1 membrane protein YoeI -
CXX03_RS10505 2081296..2082225 - 930 WP_000803351.1 LysR family transcriptional regulator -
CXX03_RS10510 2082271..2083095 - 825 WP_000754749.1 SDR family oxidoreductase -
CXX03_RS10515 2083178..2083432 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXX03_RS10520 2083429..2083680 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXX03_RS10525 2083963..2084013 + 51 WP_001364200.1 his operon leader peptide -
CXX03_RS10530 2084159..2085058 + 900 WP_001297349.1 ATP phosphoribosyltransferase -
CXX03_RS10535 2085064..2086368 + 1305 WP_001297355.1 histidinol dehydrogenase -
CXX03_RS10540 2086365..2087435 + 1071 WP_001297360.1 histidinol-phosphate transaminase -
CXX03_RS10545 2087435..2088502 + 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92332 WP_000767829.1 NZ_CP025892:c2083432-2083178 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92332 NZ_CP025892:c2083432-2083178 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92332 WP_001259255.1 NZ_CP025892:c2083680-2083429 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92332 NZ_CP025892:c2083680-2083429 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References