Detailed information of TA system TA092311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 3852601..3853203
• Replicon: chromosome
• Accession: NZ_CP025881
• Organism: Escherichia coli strain 503440

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CXX05_RS19385
• Protein ID: WP_000897305.1
• Coordinates: 3852601..3852912 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXX05_RS19390
• Protein ID: WP_000356397.1
• Coordinates: 3852913..3853203 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX05_RS19355	3847631..3848416	+	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-
CXX05_RS19360	3848515..3849114	+	600	WP_001295269.1	glucose-1-phosphatase	-
CXX05_RS19365	3849108..3849980	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CXX05_RS19370	3849977..3850414	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CXX05_RS19375	3850459..3851400	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CXX05_RS19380	3851464..3852372	-	909	WP_001162704.1	alpha/beta hydrolase	-
CXX05_RS19385	3852601..3852912	+	312	WP_000897305.1	hypothetical protein	Toxin
CXX05_RS19390	3852913..3853203	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CXX05_RS19395	3853808..3854026	+	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
CXX05_RS19400	3854245..3854487	+	243	WP_001086388.1	hypothetical protein	-
CXX05_RS19405	3854817..3855746	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CXX05_RS19410	3855743..3856378	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CXX05_RS19415	3856375..3857277	-	903	WP_001717071.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T92311 WP_000897305.1 NZ_CP025881:3852601-3852912 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T92311 NZ_CP025881:3852601-3852912 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT92311 WP_000356397.1 NZ_CP025881:3852913-3853203 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT92311 NZ_CP025881:3852913-3853203 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7