Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 4672855..4673396 Replicon chromosome
Accession NZ_CP025869
Organism Escherichia coli strain 503829

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CXX08_RS23220 Protein ID WP_000615976.1
Coordinates 4673118..4673396 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CXX08_RS23215 Protein ID WP_000729704.1
Coordinates 4672855..4673115 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX08_RS23190 4668638..4669423 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
CXX08_RS23195 4669395..4671107 + 1713 Protein_4432 flagellar biosynthesis protein FlhA -
CXX08_RS23200 4671212..4671490 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CXX08_RS23205 4671483..4671839 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
CXX08_RS23210 4671896..4672669 - 774 WP_000543895.1 C40 family peptidase -
CXX08_RS23215 4672855..4673115 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CXX08_RS23220 4673118..4673396 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CXX08_RS23225 4673552..4674292 + 741 WP_001225679.1 murein L,D-transpeptidase -
CXX08_RS23230 4674263..4675030 - 768 WP_000333380.1 class II glutamine amidotransferase -
CXX08_RS23235 4675236..4675814 - 579 WP_001716156.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4671483..4683008 11525


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T92293 WP_000615976.1 NZ_CP025869:4673118-4673396 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T92293 NZ_CP025869:4673118-4673396 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT92293 WP_000729704.1 NZ_CP025869:4672855-4673115 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT92293 NZ_CP025869:4672855-4673115 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References