Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2676599..2677101 Replicon chromosome
Accession NZ_CP025869
Organism Escherichia coli strain 503829

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXX08_RS13175 Protein ID WP_000767829.1
Coordinates 2676847..2677101 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXX08_RS13170 Protein ID WP_001259255.1
Coordinates 2676599..2676850 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX08_RS13145 2671777..2672844 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CXX08_RS13150 2672844..2673914 - 1071 WP_001297360.1 histidinol-phosphate transaminase -
CXX08_RS13155 2673911..2675215 - 1305 WP_001297355.1 histidinol dehydrogenase -
CXX08_RS13160 2675221..2676120 - 900 WP_001297349.1 ATP phosphoribosyltransferase -
CXX08_RS13165 2676266..2676316 - 51 WP_001364200.1 his operon leader peptide -
CXX08_RS13170 2676599..2676850 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXX08_RS13175 2676847..2677101 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXX08_RS13180 2677184..2678008 + 825 WP_000754749.1 SDR family oxidoreductase -
CXX08_RS13185 2678054..2678983 + 930 WP_000803351.1 LysR family transcriptional regulator -
CXX08_RS13190 2679198..2679260 + 63 WP_010723108.1 membrane protein YoeI -
CXX08_RS13195 2679250..2680608 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXX08_RS13200 2680787..2681844 + 1058 Protein_2513 transport protein YeeE -
CXX08_RS13205 2681858..2682085 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92279 WP_000767829.1 NZ_CP025869:2676847-2677101 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92279 NZ_CP025869:2676847-2677101 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92279 WP_001259255.1 NZ_CP025869:2676599-2676850 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92279 NZ_CP025869:2676599-2676850 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References