Detailed information of TA system TA092273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 602127..602729
• Replicon: chromosome
• Accession: NZ_CP025869
• Organism: Escherichia coli strain 503829

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CXX08_RS02870
• Protein ID: WP_000897305.1
• Coordinates: 602418..602729 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXX08_RS02865
• Protein ID: WP_000356397.1
• Coordinates: 602127..602417 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX08_RS02840	598053..598955	+	903	WP_001717071.1	formate dehydrogenase O subunit beta	-
CXX08_RS02845	598952..599587	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CXX08_RS02850	599584..600513	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CXX08_RS02855	600843..601085	-	243	WP_001086388.1	hypothetical protein	-
CXX08_RS02860	601304..601522	-	219	WP_001297075.1	ribbon-helix-helix domain-containing protein	-
CXX08_RS02865	602127..602417	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CXX08_RS02870	602418..602729	-	312	WP_000897305.1	hypothetical protein	Toxin
CXX08_RS02875	602958..603866	+	909	WP_001162704.1	alpha/beta hydrolase	-
CXX08_RS02880	603930..604871	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CXX08_RS02885	604916..605353	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CXX08_RS02890	605350..606222	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CXX08_RS02895	606216..606815	-	600	WP_001295269.1	glucose-1-phosphatase	-
CXX08_RS02900	606914..607699	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T92273 WP_000897305.1 NZ_CP025869:c602729-602418 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T92273 NZ_CP025869:c602729-602418 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT92273 WP_000356397.1 NZ_CP025869:c602417-602127 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT92273 NZ_CP025869:c602417-602127 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7