Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 4440834..4441336 Replicon chromosome
Accession NZ_CP025865
Organism Escherichia coli strain 504211

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXX09_RS21780 Protein ID WP_000767829.1
Coordinates 4441082..4441336 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXX09_RS21775 Protein ID WP_001259255.1
Coordinates 4440834..4441085 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX09_RS21750 4436012..4437079 - 1068 WP_000080087.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CXX09_RS21755 4437079..4438149 - 1071 WP_001297360.1 histidinol-phosphate transaminase -
CXX09_RS21760 4438146..4439450 - 1305 WP_001297355.1 histidinol dehydrogenase -
CXX09_RS21765 4439456..4440355 - 900 WP_001297349.1 ATP phosphoribosyltransferase -
CXX09_RS21770 4440501..4440551 - 51 WP_001364200.1 his operon leader peptide -
CXX09_RS21775 4440834..4441085 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXX09_RS21780 4441082..4441336 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXX09_RS21785 4441419..4442243 + 825 WP_000754749.1 SDR family oxidoreductase -
CXX09_RS21790 4442289..4443218 + 930 WP_000803351.1 LysR family transcriptional regulator -
CXX09_RS21795 4443433..4443495 + 63 WP_010723108.1 membrane protein YoeI -
CXX09_RS21800 4443485..4444843 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXX09_RS21805 4445022..4446079 + 1058 Protein_4164 transport protein YeeE -
CXX09_RS21810 4446093..4446320 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92260 WP_000767829.1 NZ_CP025865:4441082-4441336 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92260 NZ_CP025865:4441082-4441336 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92260 WP_001259255.1 NZ_CP025865:4440834-4441085 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92260 NZ_CP025865:4440834-4441085 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References