Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 1514277..1514818 Replicon chromosome
Accession NZ_CP025865
Organism Escherichia coli strain 504211

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CXX09_RS07525 Protein ID WP_000615976.1
Coordinates 1514540..1514818 (+) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CXX09_RS07520 Protein ID WP_000729704.1
Coordinates 1514277..1514537 (+) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX09_RS07495 1510060..1510845 - 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -
CXX09_RS07500 1510817..1512529 + 1713 Protein_1438 flagellar biosynthesis protein FlhA -
CXX09_RS07505 1512634..1512912 + 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CXX09_RS07510 1512905..1513261 + 357 WP_001030483.1 type II toxin-antitoxin system HicB family antitoxin -
CXX09_RS07515 1513318..1514091 - 774 WP_000543895.1 C40 family peptidase -
CXX09_RS07520 1514277..1514537 + 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CXX09_RS07525 1514540..1514818 + 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CXX09_RS07530 1514974..1515714 + 741 WP_001225679.1 murein L,D-transpeptidase -
CXX09_RS07535 1515685..1516452 - 768 WP_000333380.1 class II glutamine amidotransferase -
CXX09_RS07540 1516658..1517236 - 579 WP_001716156.1 D-sedoheptulose 7-phosphate isomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 1512905..1524430 11525


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T92250 WP_000615976.1 NZ_CP025865:1514540-1514818 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T92250 NZ_CP025865:1514540-1514818 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT92250 WP_000729704.1 NZ_CP025865:1514277-1514537 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT92250 NZ_CP025865:1514277-1514537 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References