Detailed information of TA system TA092240

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4758434..4759036
• Replicon: chromosome
• Accession: NZ_CP025862
• Organism: Escherichia coli strain 504237

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CXX10_RS23565
• Protein ID: WP_000897305.1
• Coordinates: 4758434..4758745 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXX10_RS23570
• Protein ID: WP_000356397.1
• Coordinates: 4758746..4759036 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX10_RS23535	4753464..4754249	+	786	WP_001662158.1	DeoR/GlpR family DNA-binding transcription regulator	-
CXX10_RS23540	4754348..4754947	+	600	WP_001295269.1	glucose-1-phosphatase	-
CXX10_RS23545	4754941..4755813	+	873	WP_000920762.1	virulence factor BrkB family protein	-
CXX10_RS23550	4755810..4756247	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CXX10_RS23555	4756292..4757233	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CXX10_RS23560	4757297..4758205	-	909	WP_001162704.1	alpha/beta hydrolase	-
CXX10_RS23565	4758434..4758745	+	312	WP_000897305.1	hypothetical protein	Toxin
CXX10_RS23570	4758746..4759036	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CXX10_RS23575	4759641..4759859	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CXX10_RS23580	4760078..4760320	+	243	WP_001086388.1	hypothetical protein	-
CXX10_RS23585	4760650..4761579	-	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CXX10_RS23590	4761576..4762211	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CXX10_RS23595	4762208..4763110	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T92240 WP_000897305.1 NZ_CP025862:4758434-4758745 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T92240 NZ_CP025862:4758434-4758745 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT92240 WP_000356397.1 NZ_CP025862:4758746-4759036 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT92240 NZ_CP025862:4758746-4759036 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7