Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2607995..2608497 Replicon chromosome
Accession NZ_CP025862
Organism Escherichia coli strain 504237

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXX10_RS13065 Protein ID WP_000767829.1
Coordinates 2607995..2608249 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXX10_RS13070 Protein ID WP_001259255.1
Coordinates 2608246..2608497 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX10_RS13035 2603010..2603237 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
CXX10_RS13040 2603251..2604309 - 1059 WP_000492305.1 transport protein YeeE -
CXX10_RS13045 2604488..2605846 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXX10_RS13050 2605836..2605898 - 63 WP_010723108.1 membrane protein YoeI -
CXX10_RS13055 2606113..2607042 - 930 WP_000803360.1 LysR family transcriptional regulator -
CXX10_RS13060 2607088..2607912 - 825 WP_000754737.1 SDR family oxidoreductase -
CXX10_RS13065 2607995..2608249 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXX10_RS13070 2608246..2608497 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXX10_RS13075 2608780..2608830 + 51 WP_001364200.1 his operon leader peptide -
CXX10_RS13080 2608976..2609875 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXX10_RS13085 2609881..2611185 + 1305 WP_001355813.1 histidinol dehydrogenase -
CXX10_RS13090 2611182..2612252 + 1071 WP_001099220.1 histidinol-phosphate transaminase -
CXX10_RS13095 2612252..2613319 + 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92233 WP_000767829.1 NZ_CP025862:c2608249-2607995 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92233 NZ_CP025862:c2608249-2607995 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92233 WP_001259255.1 NZ_CP025862:c2608497-2608246 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92233 NZ_CP025862:c2608497-2608246 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References