Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-RelB
Location 629747..630288 Replicon chromosome
Accession NZ_CP025862
Organism Escherichia coli strain 504237

Toxin (Protein)


Gene name yafQ Uniprot ID U9XPG0
Locus tag CXX10_RS02995 Protein ID WP_000615976.1
Coordinates 629747..630025 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID S1EWQ0
Locus tag CXX10_RS03000 Protein ID WP_000729704.1
Coordinates 630028..630288 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX10_RS02980 627329..627907 + 579 WP_000284050.1 D-sedoheptulose 7-phosphate isomerase -
CXX10_RS02985 628113..628880 + 768 WP_000333380.1 class II glutamine amidotransferase -
CXX10_RS02990 628851..629591 - 741 WP_001225679.1 murein L,D-transpeptidase -
CXX10_RS02995 629747..630025 - 279 WP_000615976.1 type II toxin-antitoxin system mRNA interferase toxin YafQ Toxin
CXX10_RS03000 630028..630288 - 261 WP_000729704.1 type II toxin-antitoxin system antitoxin DinJ Antitoxin
CXX10_RS03005 630474..631247 + 774 Protein_580 C40 family peptidase -
CXX10_RS03010 631304..631660 - 357 WP_001030484.1 type II toxin-antitoxin system HicB family antitoxin -
CXX10_RS03015 631653..631931 - 279 WP_000598760.1 type II toxin-antitoxin system HicA family toxin -
CXX10_RS03020 632036..633748 - 1713 Protein_583 flagellar biosynthesis protein FlhA -
CXX10_RS03025 633720..634505 + 786 WP_000207568.1 putative lateral flagellar export/assembly protein LafU -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(1-91)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(4-84)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10800.59 Da        Isoelectric Point: 10.0702

>T92208 WP_000615976.1 NZ_CP025862:c630025-629747 [Escherichia coli]
MIQRDIEYSGQFSKDVKLAQKRHKDMNKLKYLMTLLINNALPLPAVYKDHPLQGSWKGYRDAHVEPDWILIYKLTDKLLR
FERTGTHAALFG

Download         Length: 279 bp

>T92208 NZ_CP025862:c630025-629747 [Escherichia coli]
ATGATTCAAAGGGATATTGAATACTCGGGACAATTTTCAAAGGATGTAAAACTTGCACAAAAGCGTCATAAGGATATGAA
TAAATTGAAATATCTTATGACGCTTCTTATCAATAATGCTTTACCGCTTCCAGCTGTTTATAAAGACCACCCGCTGCAAG
GTTCATGGAAAGGTTATCGCGATGCTCATGTCGAACCGGACTGGATCCTGATTTACAAACTTACCGATAAACTTTTACGA
TTTGAGAGAACTGGAACTCACGCGGCGCTCTTTGGGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9391.77 Da        Isoelectric Point: 5.0758

>AT92208 WP_000729704.1 NZ_CP025862:c630288-630028 [Escherichia coli]
MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGVDVHKAKDADDL
FDKLGI

Download         Length: 261 bp

>AT92208 NZ_CP025862:c630288-630028 [Escherichia coli]
ATGGCTGCTAACGCATTTGTTCGCGCCCGAATCGATGAAGATCTGAAGAATCAGGCGGCGGACGTACTGGCCGGGATGGG
GCTGACCATCTCTGACCTGGTTCGCATAACCCTCACAAAGGTCGCGCGTGAAAAGGCATTGCCGTTTGATTTACGCGAGC
CTAATCAATTAACCATTCAATCAATCAAAAACAGCGAAGCTGGCGTTGATGTTCATAAGGCCAAAGACGCCGATGATTTA
TTTGATAAATTAGGAATTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9XPG0

Antitoxin

Source ID Structure
PDB 4ML0
AlphaFold DB A0A0E0Y6Z6

References