Detailed information of TA system TA092193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 2150231..2151030
• Replicon: chromosome
• Accession: NZ_CP025859
• Organism: Escherichia coli strain 504239

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: S1NYM6
• Locus tag: CXV92_RS10630
• Protein ID: WP_000347251.1
• Coordinates: 2150566..2151030 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: S1EB98
• Locus tag: CXV92_RS10625
• Protein ID: WP_001307405.1
• Coordinates: 2150231..2150566 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV92_RS10610	2146016..2146786	-	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-
CXV92_RS10615	2146802..2148136	-	1335	WP_000599636.1	galactarate/glucarate/glycerate transporter GarP	-
CXV92_RS10620	2148511..2150082	+	1572	WP_001273763.1	galactarate dehydratase	-
CXV92_RS10625	2150231..2150566	+	336	WP_001307405.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CXV92_RS10630	2150566..2151030	+	465	WP_000347251.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CXV92_RS10635	2151085..2151894	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CXV92_RS10640	2152143..2153423	+	1281	WP_000681906.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CXV92_RS10645	2153446..2153919	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CXV92_RS10650	2153930..2154709	+	780	WP_000406213.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CXV92_RS10655	2154699..2155577	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CXV92_RS10660	2155595..2156029	+	435	WP_000948827.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17777.14 Da
• Isoelectric Point: 9.4947

>T92193 WP_000347251.1 NZ_CP025859:2150566-2151030 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVEALKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWESLTQETEENH

Nucleotide

• Length: 465 bp

>T92193 NZ_CP025859:2150566-2151030 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGATGCTTTAGTTGC
CGAAGTCGAGGCATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCTGCCACAAAGTTATTGGCGGTGGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGCTATCGTCTCTTCTTTCGTTACAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGTAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAAGCCTCACCCAAGAGACAGAAGAAAACCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12358.94 Da
• Isoelectric Point: 4.8616

>AT92193 WP_001307405.1 NZ_CP025859:2150231-2150566 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT92193 NZ_CP025859:2150231-2150566 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTCTTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A829CJ20

Antitoxin

• AlphaFold DB: A0A0E0XVC7