Detailed information of TA system TA092172

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 96958..97375
• Replicon: plasmid p504838_108
• Accession: NZ_CP025857
• Organism: Escherichia coli strain 504838

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CXV93_RS26090
• Protein ID: WP_001312861.1
• Coordinates: 96958..97116 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	97181..97375 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV93_RS26065	93169..93441	+	273	WP_001695087.1	hypothetical protein	-
CXV93_RS26070	93422..94630	+	1209	WP_001695118.1	IS91 family transposase	-
CXV93_RS26075	94795..95121	-	327	WP_205755194.1	reverse transcriptase	-
CXV93_RS26085	95794..96533	-	740	Protein_118	IS3 family transposase	-
CXV93_RS26090	96958..97116	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	97181..97375	-	195	NuclAT_1	-	Antitoxin
-	97181..97375	-	195	NuclAT_1	-	Antitoxin
-	97181..97375	-	195	NuclAT_1	-	Antitoxin
-	97181..97375	-	195	NuclAT_1	-	Antitoxin
CXV93_RS26095	97387..98106	-	720	WP_001759770.1	plasmid SOS inhibition protein A	-
CXV93_RS26100	98103..98537	-	435	WP_000845924.1	conjugation system SOS inhibitor PsiB	-
CXV93_RS26105	98592..99890	-	1299	Protein_122	hypothetical protein	-
CXV93_RS26110	99889..100725	+	837	Protein_123	IS91 family transposase	-
CXV93_RS26120	101002..101367	+	366	WP_000124102.1	hypothetical protein	-
CXV93_RS26930	101352..101495	+	144	Protein_125	IS91 family transposase	-
CXV93_RS26130	101682..102295	-	614	Protein_126	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..108475	108475
	-	inside	IScluster/Tn	-	-	86766..103970	17204

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T92172 WP_001312861.1 NZ_CP025857:c97116-96958 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T92172 NZ_CP025857:c97116-96958 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 195 bp

>AT92172 NZ_CP025857:c97375-97181 [Escherichia coli]
TCACGCGGATTTCCCGTAGCCTGAATGAGCGTATTCTTTTCAGGAAAAGTGAATGTGGCCAGCGCGCAGGGATATGGGCT
ATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACAGTGTTGTGTGGCAGAAGGACAAA
AGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin