Detailed information of TA system TA092167

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 49160..49586
• Replicon: plasmid p504838_108
• Accession: NZ_CP025857
• Organism: Escherichia coli strain 504838

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CXV93_RS25730
• Protein ID: WP_001312861.1
• Coordinates: 49160..49318 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	49362..49586 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXV93_RS25695	44557..45246	-	690	WP_000283387.1	conjugal transfer transcriptional regulator TraJ	-
CXV93_RS25700	45433..45816	-	384	WP_001151538.1	relaxosome protein TraM	-
CXV93_RS25705	46230..46739	+	510	WP_000759173.1	transglycosylase SLT domain-containing protein	-
CXV93_RS25710	47036..47857	-	822	WP_001234445.1	DUF945 domain-containing protein	-
CXV93_RS25715	47967..48263	-	297	WP_001272251.1	hypothetical protein	-
CXV93_RS25725	48655..48859	+	205	Protein_58	pilus protein	-
CXV93_RS25730	49160..49318	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	49362..49586	-	225	NuclAT_0	-	Antitoxin
-	49362..49586	-	225	NuclAT_0	-	Antitoxin
-	49362..49586	-	225	NuclAT_0	-	Antitoxin
-	49362..49586	-	225	NuclAT_0	-	Antitoxin
CXV93_RS26915	49398..49577	+	180	WP_001309233.1	hypothetical protein	-
CXV93_RS25735	49598..50317	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
CXV93_RS25740	50314..50748	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CXV93_RS25745	50817..52781	-	1965	WP_032327613.1	ParB/RepB/Spo0J family partition protein	-
CXV93_RS25750	52842..53075	-	234	WP_000006003.1	DUF905 family protein	-
CXV93_RS25755	53133..53660	-	528	WP_000290834.1	single-stranded DNA-binding protein	-
CXV93_RS27050	54054..54447	+	394	Protein_66	pilus protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	cofA	1..108475	108475

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T92167 WP_001312861.1 NZ_CP025857:c49318-49160 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T92167 NZ_CP025857:c49318-49160 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT92167 NZ_CP025857:c49586-49362 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin