Detailed information of TA system TA092133

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 27193..27447
• Replicon: plasmid p510016_29
• Accession: NZ_CP025852
• Organism: Escherichia coli strain 510016

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CXX11_RS24705
• Protein ID: WP_001362723.1
• Coordinates: 27241..27447 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	27193..27249 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX11_RS24675	23736..24011	+	276	Protein_25	pilin	-
CXX11_RS24680	24035..25231	+	1197	WP_001401520.1	DNA helicase TraI family protein	-
CXX11_RS24685	25249..25815	+	567	WP_024169954.1	DUF2726 domain-containing protein	-
CXX11_RS24690	25812..26543	+	732	WP_001401518.1	type-F conjugative transfer system pilin acetylase TraX	-
CXX11_RS24695	26767..26949	-	183	WP_001401517.1	hypothetical protein	-
-	27193..27249	-	57	NuclAT_0	-	Antitoxin
-	27193..27249	-	57	NuclAT_0	-	Antitoxin
-	27193..27249	-	57	NuclAT_0	-	Antitoxin
-	27193..27249	-	57	NuclAT_0	-	Antitoxin
CXX11_RS24705	27241..27447	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
CXX11_RS24710	27731..27991	+	261	WP_000083817.1	replication regulatory protein RepA	-
CXX11_RS24720	28214..28288	+	75	WP_001365571.1	RepA leader peptide Tap	-
CXX11_RS24725	28281..29138	+	858	WP_001401515.1	incFII family plasmid replication initiator RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..29775	29775

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T92133 WP_001362723.1 NZ_CP025852:27241-27447 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T92133 NZ_CP025852:27241-27447 [Escherichia coli]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACTTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT92133 NZ_CP025852:c27249-27193 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin