Detailed information of TA system TA092129

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3611879..3612573
• Replicon: chromosome
• Accession: NZ_CP025851
• Organism: Escherichia coli strain 510016

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CXX11_RS17960
• Protein ID: WP_001263493.1
• Coordinates: 3612175..3612573 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: -
• Locus tag: CXX11_RS17955
• Protein ID: WP_001399937.1
• Coordinates: 3611879..3612172 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX11_RS17935	3607519..3608016	+	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-
CXX11_RS17940	3608232..3609944	-	1713	Protein_3426	flagellar biosynthesis protein FlhA	-
CXX11_RS17945	3609916..3610701	+	786	WP_000207564.1	putative lateral flagellar export/assembly protein LafU	-
CXX11_RS17950	3610772..3611827	+	1056	WP_001399936.1	DNA polymerase IV	-
CXX11_RS17955	3611879..3612172	+	294	WP_001399937.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CXX11_RS17960	3612175..3612573	+	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CXX11_RS17965	3612583..3612693	+	111	Protein_3431	GNAT family N-acetyltransferase	-
CXX11_RS17970	3612710..3613407	-	698	Protein_3432	IS1 family transposase	-
CXX11_RS17975	3613515..3613718	+	204	Protein_3433	RNA ligase RtcB family protein	-
CXX11_RS17980	3613717..3614238	+	522	Protein_3434	peptide chain release factor H	-
CXX11_RS17985	3614295..3615752	-	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CXX11_RS17990	3616013..3616471	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
-	3617067..3617147	+	81	NuclAT_11	-	-
-	3617067..3617147	+	81	NuclAT_11	-	-
-	3617067..3617147	+	81	NuclAT_11	-	-
-	3617067..3617147	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3612710..3613213	503

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T92129 WP_001263493.1 NZ_CP025851:3612175-3612573 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T92129 NZ_CP025851:3612175-3612573 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11267.78 Da
• Isoelectric Point: 5.2170

>AT92129 WP_001399937.1 NZ_CP025851:3611879-3612172 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARFEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT92129 NZ_CP025851:3611879-3612172 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTTGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8