Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3311070..3311572 Replicon chromosome
Accession NZ_CP025849
Organism Escherichia coli strain 600468

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXX14_RS15840 Protein ID WP_000767829.1
Coordinates 3311318..3311572 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXX14_RS15835 Protein ID WP_001259255.1
Coordinates 3311070..3311321 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX14_RS15810 3306248..3307315 - 1068 WP_000080060.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CXX14_RS15815 3307315..3308385 - 1071 WP_000108941.1 histidinol-phosphate transaminase -
CXX14_RS15820 3308382..3309686 - 1305 WP_001317939.1 histidinol dehydrogenase -
CXX14_RS15825 3309692..3310591 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXX14_RS15830 3310737..3310787 - 51 WP_001364200.1 his operon leader peptide -
CXX14_RS15835 3311070..3311321 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXX14_RS15840 3311318..3311572 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXX14_RS15845 3311655..3312479 + 825 WP_000754763.1 SDR family oxidoreductase -
CXX14_RS15850 3312525..3313454 + 930 WP_000803351.1 LysR family transcriptional regulator -
CXX14_RS15855 3313669..3313731 + 63 WP_010723108.1 membrane protein YoeI -
CXX14_RS15860 3313721..3315079 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXX14_RS15865 3315258..3316316 + 1059 WP_000492335.1 transport protein YeeE -
CXX14_RS15870 3316330..3316557 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92101 WP_000767829.1 NZ_CP025849:3311318-3311572 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92101 NZ_CP025849:3311318-3311572 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92101 WP_001259255.1 NZ_CP025849:3311070-3311321 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92101 NZ_CP025849:3311070-3311321 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References