Detailed information of TA system TA092095

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 1262803..1263405
• Replicon: chromosome
• Accession: NZ_CP025849
• Organism: Escherichia coli strain 600468

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CXX14_RS05875
• Protein ID: WP_000897305.1
• Coordinates: 1263094..1263405 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CXX14_RS05870
• Protein ID: WP_000356397.1
• Coordinates: 1262803..1263093 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX14_RS05845	1258728..1259630	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
CXX14_RS05850	1259627..1260262	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CXX14_RS05855	1260259..1261188	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
CXX14_RS05860	1261518..1261760	-	243	WP_001087409.1	hypothetical protein	-
CXX14_RS05865	1261980..1262198	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
CXX14_RS05870	1262803..1263093	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
CXX14_RS05875	1263094..1263405	-	312	WP_000897305.1	hypothetical protein	Toxin
CXX14_RS05880	1263634..1264542	+	909	WP_001162704.1	alpha/beta hydrolase	-
CXX14_RS05885	1264606..1265547	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CXX14_RS05890	1265592..1266029	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CXX14_RS05895	1266026..1266898	-	873	WP_000920762.1	virulence factor BrkB family protein	-
CXX14_RS05900	1266892..1267491	-	600	WP_001295269.1	glucose-1-phosphatase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T92095 WP_000897305.1 NZ_CP025849:c1263405-1263094 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T92095 NZ_CP025849:c1263405-1263094 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTTTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTATTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT92095 WP_000356397.1 NZ_CP025849:c1263093-1262803 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT92095 NZ_CP025849:c1263093-1262803 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7