Detailed information of TA system TA092027

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 131437..131691
• Replicon: plasmid p214_4_132
• Accession: NZ_CP025841
• Organism: Escherichia coli strain 214-4

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: CXX41_RS26455
• Protein ID: WP_001362723.1
• Coordinates: 131485..131691 (+)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	131437..131493 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX41_RS26430	126639..129476	+	2838	Protein_155	AAA family ATPase	-
CXX41_RS26435	129494..130059	+	566	Protein_156	DUF2726 domain-containing protein	-
CXX41_RS26440	130056..130787	+	732	WP_001595201.1	type-F conjugative transfer system pilin acetylase TraX	-
CXX41_RS26445	131011..131193	-	183	WP_001393357.1	hypothetical protein	-
-	131437..131493	-	57	NuclAT_0	-	Antitoxin
-	131437..131493	-	57	NuclAT_0	-	Antitoxin
-	131437..131493	-	57	NuclAT_0	-	Antitoxin
-	131437..131493	-	57	NuclAT_0	-	Antitoxin
CXX41_RS26455	131485..131691	+	207	WP_001362723.1	type I toxin-antitoxin system Hok family toxin	Toxin
CXX41_RS26460	131975..132235	+	261	WP_000083817.1	replication regulatory protein RepA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	cssA / cofA / faeD / faeE / estIa	1..132527	132527

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7792.29 Da
• Isoelectric Point: 8.8807

>T92027 WP_001362723.1 NZ_CP025841:131485-131691 [Escherichia coli]
MKYLNTTDCSLFLAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T92027 NZ_CP025841:131485-131691 [Escherichia coli]
ATGAAGTACTTGAACACTACTGATTGTAGCCTCTTCCTTGCAGAGAGGTCAAAGTTTATGACGAAATATGCCCTTATCGG
GTTGCTCGCCGTGTGTGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTTATGTGAACTGAATATTCACAGAG
GAAATACAGTGGTGCAGGTAACCTTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 57 bp

>AT92027 NZ_CP025841:c131493-131437 [Escherichia coli]
GTACTTCATGCAGACCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCCTTTCT

Structures

Antitoxin