Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3744506..3745008 Replicon chromosome
Accession NZ_CP025840
Organism Escherichia coli strain 214-4

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CXX41_RS18345 Protein ID WP_000767829.1
Coordinates 3744754..3745008 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CXX41_RS18340 Protein ID WP_001259255.1
Coordinates 3744506..3744757 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CXX41_RS18315 3739684..3740751 - 1068 WP_000080077.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CXX41_RS18320 3740751..3741821 - 1071 WP_001099220.1 histidinol-phosphate transaminase -
CXX41_RS18325 3741818..3743122 - 1305 WP_001355813.1 histidinol dehydrogenase -
CXX41_RS18330 3743128..3744027 - 900 WP_000131782.1 ATP phosphoribosyltransferase -
CXX41_RS18335 3744173..3744223 - 51 WP_001364200.1 his operon leader peptide -
CXX41_RS18340 3744506..3744757 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CXX41_RS18345 3744754..3745008 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CXX41_RS18350 3745091..3745915 + 825 WP_000754737.1 SDR family oxidoreductase -
CXX41_RS18355 3745961..3746890 + 930 WP_000803360.1 LysR family transcriptional regulator -
CXX41_RS18360 3747105..3747167 + 63 WP_010723108.1 membrane protein YoeI -
CXX41_RS18365 3747157..3748515 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CXX41_RS18370 3748694..3749752 + 1059 WP_000492305.1 transport protein YeeE -
CXX41_RS18375 3749766..3749993 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T92014 WP_000767829.1 NZ_CP025840:3744754-3745008 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T92014 NZ_CP025840:3744754-3745008 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT92014 WP_001259255.1 NZ_CP025840:3744506-3744757 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT92014 NZ_CP025840:3744506-3744757 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References