Detailed information of TA system TA092005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 699201..699895
• Replicon: chromosome
• Accession: NZ_CP025840
• Organism: Escherichia coli strain 214-4

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: S1EXB8
• Locus tag: CXX41_RS03610
• Protein ID: WP_001263493.1
• Coordinates: 699201..699599 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1FJN6
• Locus tag: CXX41_RS03615
• Protein ID: WP_000554757.1
• Coordinates: 699602..699895 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CXX41_RS03580	694221..695465	-	1245	WP_000189541.1	esterase FrsA	-
-	694881..694961	-	81	NuclAT_11	-	-
-	694881..694961	-	81	NuclAT_11	-	-
-	694881..694961	-	81	NuclAT_11	-	-
-	694881..694961	-	81	NuclAT_11	-	-
CXX41_RS03585	695557..696015	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
CXX41_RS03590	696276..697733	+	1458	WP_001293003.1	cytosol nonspecific dipeptidase	-
CXX41_RS03595	697790..698302	-	513	Protein_691	peptide chain release factor H	-
CXX41_RS03600	698308..698493	-	186	Protein_692	RNA ligase RtcB family protein	-
CXX41_RS03605	698739..699191	-	453	WP_001059847.1	GNAT family N-acetyltransferase	-
CXX41_RS03610	699201..699599	-	399	WP_001263493.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
CXX41_RS03615	699602..699895	-	294	WP_000554757.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
CXX41_RS03620	699947..701002	-	1056	WP_001226164.1	DNA polymerase IV	-
CXX41_RS03625	701073..701858	-	786	WP_000207549.1	putative lateral flagellar export/assembly protein LafU	-
CXX41_RS03630	701830..703542	+	1713	Protein_698	flagellar biosynthesis protein FlhA	-
CXX41_RS03635	703758..704255	-	498	WP_000006261.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15472.83 Da
• Isoelectric Point: 8.0949

>T92005 WP_001263493.1 NZ_CP025840:c699599-699201 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKQARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T92005 NZ_CP025840:c699599-699201 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGTGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACAGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.81 Da
• Isoelectric Point: 5.9050

>AT92005 WP_000554757.1 NZ_CP025840:c699895-699602 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EKYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT92005 NZ_CP025840:c699895-699602 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATTACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGAGCT
GAAAAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: S1EXB8

Antitoxin

• AlphaFold DB: S1FJN6