Detailed information of TA system TA091944

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1349438..1350000
• Replicon: chromosome
• Accession: NZ_CP025757
• Organism: Citrobacter freundii complex sp. CFNIH2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A3S7DBL2
• Locus tag: WM46_RS06810
• Protein ID: WP_043000694.1
• Coordinates: 1349722..1350000 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: WM46_RS06805
• Protein ID: WP_102601809.1
• Coordinates: 1349438..1349722 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
WM46_RS06790	1347131..1348015	+	885	WP_102601807.1	formate dehydrogenase subunit beta	-
WM46_RS06795	1348008..1348664	+	657	WP_102601808.1	formate dehydrogenase-N subunit gamma	-
WM46_RS06800	1348791..1349393	+	603	WP_061077525.1	inorganic diphosphatase	-
WM46_RS06805	1349438..1349722	-	285	WP_102601809.1	HigA family addiction module antidote protein	Antitoxin
WM46_RS06810	1349722..1350000	-	279	WP_043000694.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
WM46_RS06815	1350180..1351895	-	1716	WP_102601810.1	ATP-binding cassette domain-containing protein	-
WM46_RS06820	1352204..1353901	-	1698	WP_061077522.1	malate dehydrogenase	-
WM46_RS06825	1354073..1354216	-	144	WP_061077521.1	stationary-phase-induced ribosome-associated protein	-
WM46_RS06830	1354444..1354875	+	432	WP_061077520.1	peroxiredoxin OsmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-83)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10573.12 Da
• Isoelectric Point: 8.9461

>T91944 WP_043000694.1 NZ_CP025757:c1350000-1349722 [Citrobacter freundii complex sp. CFNIH2]
MIMNFRHKGLRDLFLHGKTSGVLAKHVKRLRHRLAVIDAAGCVNDINMPGYRLHPLSGDRDGVWSISVSGNWRMTFEFVN
GDAYILDYEDYH

Nucleotide

• Length: 279 bp

>T91944 NZ_CP025757:c1350000-1349722 [Citrobacter freundii complex sp. CFNIH2]
ATGATCATGAATTTCAGGCACAAGGGATTACGCGACCTGTTTCTTCACGGTAAAACGTCAGGCGTGCTGGCAAAGCACGT
CAAACGTTTGCGACACCGCCTCGCCGTGATTGACGCCGCAGGCTGCGTGAACGATATCAACATGCCCGGTTACCGACTGC
ATCCCCTGAGCGGCGATCGTGATGGCGTCTGGTCAATATCGGTTTCAGGCAACTGGCGTATGACGTTCGAATTCGTCAAT
GGCGATGCGTACATTCTGGATTACGAGGATTATCACTGA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10384.10 Da
• Isoelectric Point: 10.2982

>AT91944 WP_102601809.1 NZ_CP025757:c1349722-1349438 [Citrobacter freundii complex sp. CFNIH2]
MKMANHPRPGDIIQEALDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPEMWLNLQNTWSLAQAQ
KSVDISRLRRLAVQ

Nucleotide

• Length: 285 bp

>AT91944 NZ_CP025757:c1349722-1349438 [Citrobacter freundii complex sp. CFNIH2]
ATGAAAATGGCGAATCATCCCCGCCCCGGGGATATCATTCAGGAAGCGCTGGACGAACTGAACGTCAGTTTGCGTGAATT
TGCCAGAGCAATGGAGATTGCGCCCTCAACCGCCAGCCGGTTACTCACGGGCAAAGCGGCACTCACCCCGGAAATGGCGA
TCAAACTTTCGGTGGTGATCGGCAGTTCCCCGGAAATGTGGCTGAATCTGCAAAATACCTGGAGTCTGGCGCAAGCGCAA
AAAAGCGTAGACATTTCCCGCCTGCGCCGTCTGGCGGTGCAGTAG

Structures

Toxin

• AlphaFold DB: A0A3S7DBL2