Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 60266..60801 Replicon plasmid pEFA-99d7
Accession NZ_CP025756
Organism Enterococcus faecium strain AALTL

Toxin (Protein)


Gene name yoeB Uniprot ID Q3Y221
Locus tag BXT96_RS15040 Protein ID WP_002305108.1
Coordinates 60266..60538 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A133CED7
Locus tag BXT96_RS15045 Protein ID WP_002303154.1
Coordinates 60538..60801 (-) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BXT96_RS15010 55272..56459 - 1188 WP_002303156.1 NADP-dependent malic enzyme -
BXT96_RS15015 56647..57594 + 948 WP_002303155.1 DNA-binding transcriptional regulator -
BXT96_RS15020 57891..58439 - 549 WP_002292681.1 hypothetical protein -
BXT96_RS15025 58440..59297 - 858 WP_002292680.1 AAA family ATPase -
BXT96_RS15030 59583..59870 - 288 WP_002300557.1 type II toxin-antitoxin system RelE/ParE family toxin -
BXT96_RS15035 59860..60189 - 330 WP_002292678.1 AbrB family transcriptional regulator -
BXT96_RS15040 60266..60538 - 273 WP_002305108.1 Txe/YoeB family addiction module toxin Toxin
BXT96_RS15045 60538..60801 - 264 WP_002303154.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
BXT96_RS15050 60907..61323 - 417 WP_002303153.1 hypothetical protein -
BXT96_RS15055 61329..61892 - 564 Protein_69 IS6 family transposase -
BXT96_RS15060 61972..63134 + 1163 WP_102642289.1 IS3 family transposase -
BXT96_RS15065 63606..64505 - 900 WP_002303235.1 aldose 1-epimerase family protein -
BXT96_RS15070 64526..64798 - 273 WP_002305107.1 DUF3884 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aac(6')-aph(2'') - 1..258454 258454


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10806.28 Da        Isoelectric Point: 9.9080

>T91933 WP_002305108.1 NZ_CP025756:c60538-60266 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTYEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T91933 NZ_CP025756:c60538-60266 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACATATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9886.16 Da        Isoelectric Point: 4.5370

>AT91933 WP_002303154.1 NZ_CP025756:c60801-60538 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDLKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT91933 NZ_CP025756:c60801-60538 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTTGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A133CER3

Antitoxin

Source ID Structure
AlphaFold DB A0A133CED7

References