Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 3874136..3874638 Replicon chromosome
Accession NZ_CP025753
Organism Escherichia coli strain CV839-06

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CV83906_RS20785 Protein ID WP_000767829.1
Coordinates 3874384..3874638 (+) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CV83906_RS20780 Protein ID WP_001259255.1
Coordinates 3874136..3874387 (+) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CV83906_RS20750 3869314..3870381 - 1068 WP_000080097.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -
CV83906_RS20755 3870381..3871451 - 1071 WP_000108937.1 histidinol-phosphate transaminase -
CV83906_RS20760 3871448..3872752 - 1305 WP_001372394.1 histidinol dehydrogenase -
CV83906_RS20765 3872758..3873657 - 900 WP_000131776.1 ATP phosphoribosyltransferase -
CV83906_RS20775 3873803..3873853 - 51 WP_001364200.1 his operon leader peptide -
CV83906_RS20780 3874136..3874387 + 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CV83906_RS20785 3874384..3874638 + 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CV83906_RS20790 3874721..3875545 + 825 WP_000754749.1 SDR family oxidoreductase -
CV83906_RS20795 3875591..3876520 + 930 WP_000803354.1 LysR family transcriptional regulator -
CV83906_RS20800 3876735..3876797 + 63 WP_010723108.1 membrane protein YoeI -
CV83906_RS20805 3876787..3878145 + 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CV83906_RS20810 3878314..3879372 + 1059 WP_000492347.1 transport protein YeeE -
CV83906_RS20815 3879386..3879613 + 228 WP_000985273.1 sulfurtransferase TusA family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T91919 WP_000767829.1 NZ_CP025753:3874384-3874638 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T91919 NZ_CP025753:3874384-3874638 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT91919 WP_001259255.1 NZ_CP025753:3874136-3874387 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT91919 NZ_CP025753:3874136-3874387 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References