Detailed information of TA system TA091902

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 72257..72521
• Replicon: plasmid pCV839-06-p1
• Accession: NZ_CP025751
• Organism: Escherichia coli strain CV839-06

Toxin (Protein)

• Gene name: pndA
• Uniprot ID: E6BRV3
• Locus tag: CV83906_RS00495
• Protein ID: WP_001303307.1
• Coordinates: 72369..72521 (+)
• Length: 51 a.a.

Antitoxin (RNA)

Gene name	pndB
Locus tag	-
Coordinates	72257..72319 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CV83906_RS00480	68359..69429	-	1071	WP_000151582.1	IncI1-type conjugal transfer protein TrbB	-
CV83906_RS00485	69448..70656	-	1209	WP_000121273.1	IncI1-type conjugal transfer protein TrbA	-
-	70836..70896	-	61	NuclAT_1	-	-
-	70836..70896	-	61	NuclAT_1	-	-
-	70836..70896	-	61	NuclAT_1	-	-
-	70836..70896	-	61	NuclAT_1	-	-
CV83906_RS00490	70963..72054	-	1092	WP_000426061.1	hypothetical protein	-
-	72257..72319	-	63	NuclAT_0	-	Antitoxin
-	72257..72319	-	63	NuclAT_0	-	Antitoxin
-	72257..72319	-	63	NuclAT_0	-	Antitoxin
-	72257..72319	-	63	NuclAT_0	-	Antitoxin
CV83906_RS00495	72369..72521	+	153	WP_001303307.1	Hok/Gef family protein	Toxin
CV83906_RS00500	72593..72844	-	252	WP_001291965.1	hypothetical protein	-
-	73231..73282	-	52	NuclAT_2	-	-
-	73231..73282	-	52	NuclAT_2	-	-
-	73231..73282	-	52	NuclAT_2	-	-
-	73231..73282	-	52	NuclAT_2	-	-
CV83906_RS26765	73768..73944	-	177	WP_001054900.1	hypothetical protein	-
CV83906_RS00515	74153..74362	-	210	Protein_84	hemolysin expression modulator Hha	-
CV83906_RS00520	74460..75074	-	615	WP_000578649.1	plasmid IncI1-type surface exclusion protein ExcA	-
CV83906_RS00525	75150..77318	-	2169	WP_000698360.1	IncI1-type conjugal transfer membrane protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	aph(6)-Id / aph(3'')-Ib / tet(B)	-	1..110160	110160

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (7-46)

Sequences

Toxin

Protein

• Length: 51 a.a.
• Molecular weight: 5817.21 Da
• Isoelectric Point: 8.7948

>T91902 WP_001303307.1 NZ_CP025751:72369-72521 [Escherichia coli]
MPQRTFLMMLIVICVTILCFVWMVRDSLCVLRLQQGNTVLVATLAYEVKR

Nucleotide

• Length: 153 bp

>T91902 NZ_CP025751:72369-72521 [Escherichia coli]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCATCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGTACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin

Nucleotide

• Length: 63 bp

>AT91902 NZ_CP025751:c72319-72257 [Escherichia coli]
TCGAAGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Structures

Toxin

• AlphaFold DB: A0A634Q5L1

Antitoxin