Detailed information of TA system TA091895

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3866714..3867408
• Replicon: chromosome
• Accession: NZ_CP025747
• Organism: Escherichia coli strain ML35

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: -
• Locus tag: C1I23_RS20545
• Protein ID: WP_102597213.1
• Coordinates: 3866714..3867112 (-)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: C1I23_RS20550
• Protein ID: WP_000554758.1
• Coordinates: 3867115..3867408 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
-	3862302..3862382	-	81	NuclAT_13	-	-
-	3862302..3862382	-	81	NuclAT_13	-	-
-	3862302..3862382	-	81	NuclAT_13	-	-
-	3862302..3862382	-	81	NuclAT_13	-	-
C1I23_RS20515	3862978..3863436	-	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
C1I23_RS20520	3863697..3865154	+	1458	WP_001292994.1	cytosol nonspecific dipeptidase	-
C1I23_RS20525	3865211..3865732	-	522	Protein_3757	peptide chain release factor H	-
C1I23_RS20530	3865728..3865934	-	207	Protein_3758	RtcB family protein	-
C1I23_RS20540	3866252..3866704	-	453	WP_001059892.1	GNAT family N-acetyltransferase	-
C1I23_RS20545	3866714..3867112	-	399	WP_102597213.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
C1I23_RS20550	3867115..3867408	-	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
C1I23_RS20555	3867460..3868515	-	1056	WP_001226164.1	DNA polymerase IV	-
C1I23_RS20560	3868586..3869371	-	786	WP_000207552.1	putative lateral flagellar export/assembly protein LafU	-
C1I23_RS20565	3869343..3871055	+	1713	Protein_3764	flagellar biosynthesis protein FlhA	-
C1I23_RS20570	3871279..3871776	-	498	WP_000006255.1	REP-associated tyrosine transposase RayT	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	gmhA/lpcA / rhs/PAAR / tssA / hcp1/tssD1	3866714..3894922	28208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15521.86 Da
• Isoelectric Point: 7.4215

>T91895 WP_102597213.1 NZ_CP025747:c3867112-3866714 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLFIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T91895 NZ_CP025747:c3867112-3866714 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGTTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT91895 WP_000554758.1 NZ_CP025747:c3867408-3867115 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT91895 NZ_CP025747:c3867408-3867115 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: S1QAE3