Detailed information of TA system TA091889

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-ralA/-
• Location: 2979829..2980250
• Replicon: chromosome
• Accession: NZ_CP025747
• Organism: Escherichia coli strain ML35

Toxin (Protein)

• Gene name: hok
• Uniprot ID: B1VC78
• Locus tag: C1I23_RS16055
• Protein ID: WP_001302184.1
• Coordinates: 2980092..2980250 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2979829..2980027 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1I23_RS26320	2974849..2975619	+	771	WP_199766327.1	hypothetical protein	-
C1I23_RS16015	2975923..2976225	+	303	WP_122998873.1	antirestriction protein	-
C1I23_RS16020	2976272..2976694	+	423	WP_024239105.1	DUF1380 family protein	-
C1I23_RS16025	2976691..2976882	+	192	WP_001027495.1	hypothetical protein	-
C1I23_RS26325	2976852..2977363	-	512	Protein_2942	hypothetical protein	-
C1I23_RS16040	2977681..2978133	+	453	WP_021569034.1	single-stranded DNA-binding protein	-
C1I23_RS16045	2978190..2978423	+	234	WP_024239188.1	DUF905 family protein	-
C1I23_RS16050	2978489..2979860	+	1372	Protein_2945	plasmid SOS inhibition protein A	-
-	2979829..2980027	+	199	NuclAT_1	-	Antitoxin
-	2979829..2980027	+	199	NuclAT_1	-	Antitoxin
-	2979829..2980027	+	199	NuclAT_1	-	Antitoxin
-	2979829..2980027	+	199	NuclAT_1	-	Antitoxin
C1I23_RS16055	2980092..2980250	+	159	WP_001302184.1	type I toxin-antitoxin system Hok family toxin	Toxin
C1I23_RS26330	2980918..2981124	+	207	WP_000547965.1	hypothetical protein	-
C1I23_RS16065	2981149..2981436	+	288	WP_021569031.1	hypothetical protein	-
C1I23_RS16070	2981555..2982376	+	822	WP_024239203.1	DUF945 domain-containing protein	-
C1I23_RS16075	2982671..2983181	-	511	Protein_2950	transglycosylase SLT domain-containing protein	-
C1I23_RS16080	2983594..2983977	+	384	WP_021569028.1	relaxosome protein TraM	-
C1I23_RS16085	2984164..2984616	+	453	Protein_2952	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2961769..3028812	67043
	-	inside	IScluster/Tn	-	-	2984832..2999148	14316

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6108.35 Da
• Isoelectric Point: 9.1977

>T91889 WP_001302184.1 NZ_CP025747:2980092-2980250 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGYREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T91889 NZ_CP025747:2980092-2980250 [Escherichia coli]
ATGAAACTACCACGCAGCTCTCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGATACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 199 bp

>AT91889 NZ_CP025747:2979829-2980027 [Escherichia coli]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGGATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAGGA
CAAAAGCCCCGTAGTTAATTTTTCATTAACCCACGAGGC

Structures

Antitoxin