Detailed information of TA system TA091874

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): ralRA/-
• Location: 2275501..2275872
• Replicon: chromosome
• Accession: NZ_CP025747
• Organism: Escherichia coli strain ML35

Toxin (Protein)

• Gene name: ralR
• Uniprot ID: A0A839BBC2
• Locus tag: C1I23_RS12270
• Protein ID: WP_021500490.1
• Coordinates: 2275678..2275872 (-)
• Length: 65 a.a.

Antitoxin (RNA)

Gene name	ralA
Locus tag	-
Coordinates	2275501..2275679 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C1I23_RS12245	2271455..2272828	+	1374	WP_000123737.1	ATP-dependent RNA helicase DbpA	-
C1I23_RS12250	2272957..2273892	-	936	WP_001157376.1	tRNA 2-thiocytidine(32) synthetase TtcA	-
C1I23_RS12255	2273945..2275180	-	1236	WP_000040838.1	site-specific integrase	-
C1I23_RS12260	2275182..2275397	-	216	WP_000079604.1	excisionase XisR	-
C1I23_RS12265	2275497..2275685	-	189	WP_001298826.1	DUF1187 family protein	-
-	2275501..2275679	+	179	NuclAT_0	-	Antitoxin
-	2275501..2275679	+	179	NuclAT_0	-	Antitoxin
-	2275501..2275679	+	179	NuclAT_0	-	Antitoxin
-	2275501..2275679	+	179	NuclAT_0	-	Antitoxin
C1I23_RS12270	2275678..2275872	-	195	WP_021500490.1	type I toxin-antitoxin system endodeoxyribonuclease toxin RalR	Toxin
C1I23_RS12275	2275936..2276988	-	1053	WP_001004423.1	recombinase RecT	-
C1I23_RS12280	2277000..2280125	-	3126	WP_000102216.1	exodeoxyribonuclease VIII	-
C1I23_RS12285	2280227..2280501	-	275	Protein_2252	hypothetical protein	-
C1I23_RS12290	2280576..2280752	-	177	WP_000245528.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2153448..2315298	161850
	-	inside	Prophage	-	-	2243938..2315298	71360

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Endodeoxyribonuclease toxin RalR (6-54)

Sequences

Toxin

Protein

• Length: 65 a.a.
• Molecular weight: 7046.98 Da
• Isoelectric Point: 9.2886

>T91874 WP_021500490.1 NZ_CP025747:c2275872-2275678 [Escherichia coli]
MRYEKVKPCPFCGCPSVTVKAISGYYRAKCNGCESRTGYGGSEKEALERWNKRTTGNNNGGVHV

Nucleotide

• Length: 195 bp

>T91874 NZ_CP025747:c2275872-2275678 [Escherichia coli]
ATGCGGTATGAAAAAGTCAAACCATGCCCTTTTTGTGGTTGTCCATCAGTAACGGTGAAAGCCATTTCAGGATATTACCG
CGCGAAGTGTAACGGATGCGAATCCCGAACCGGCTATGGTGGAAGTGAAAAAGAAGCACTCGAACGATGGAATAAACGAA
CCACTGGAAATAATAATGGAGGTGTTCATGTATAA

Antitoxin

Nucleotide

• Length: 179 bp

>AT91874 NZ_CP025747:2275501-2275679 [Escherichia coli]
GAGGACTGAAGTTGCTCGCAATTAAAATTTATCAGTTTTACTTTCTGCTCTCTGGAAACGCCTGCTTCTTTTTTACCTGA
GAGCATTTTTTCGCATTCTGATTTGGTTAATTTTGTTTTTGAGTACCTTGTCCAGTTAGTAGGAGTGCCACCTTCCTTTT
CAATAGTGGCGGTAATTTT

Structures

Toxin

• AlphaFold DB: A0A839BBC2

Antitoxin