Detailed information of TA system TA091851

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4335899..4336501
• Replicon: chromosome
• Accession: NZ_CP025716
• Organism: Escherichia coli strain BH100L substr. MG2017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BH100L_RS21910
• Protein ID: WP_000897305.1
• Coordinates: 4335899..4336210 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BH100L_RS21915
• Protein ID: WP_000356397.1
• Coordinates: 4336211..4336501 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100L_RS21885	4331813..4332412	+	600	WP_001298594.1	glucose-1-phosphatase	-
BH100L_RS21890	4332406..4333278	+	873	WP_000920762.1	virulence factor BrkB family protein	-
BH100L_RS21895	4333275..4333712	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BH100L_RS21900	4333757..4334698	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BH100L_RS21905	4334762..4335670	-	909	WP_001298596.1	alpha/beta hydrolase	-
BH100L_RS21910	4335899..4336210	+	312	WP_000897305.1	hypothetical protein	Toxin
BH100L_RS21915	4336211..4336501	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BH100L_RS21925	4337106..4337324	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
BH100L_RS21930	4337539..4338468	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
BH100L_RS21935	4338465..4339100	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BH100L_RS21940	4339097..4339999	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T91851 WP_000897305.1 NZ_CP025716:4335899-4336210 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T91851 NZ_CP025716:4335899-4336210 [Escherichia coli]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT91851 WP_000356397.1 NZ_CP025716:4336211-4336501 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT91851 NZ_CP025716:4336211-4336501 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7