Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 2114528..2115030 Replicon chromosome
Accession NZ_CP025716
Organism Escherichia coli strain BH100L substr. MG2017

Toxin (Protein)


Gene name yoeB Uniprot ID S1P977
Locus tag BH100L_RS10835 Protein ID WP_000767822.1
Coordinates 2114528..2114782 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag BH100L_RS10840 Protein ID WP_001259255.1
Coordinates 2114779..2115030 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH100L_RS10815 2111021..2112379 - 1359 WP_000019194.1 putrescine/proton symporter PlaP -
BH100L_RS10820 2112369..2112431 - 63 WP_010723108.1 membrane protein YoeI -
BH100L_RS10825 2112646..2113575 - 930 WP_000803366.1 LysR family transcriptional regulator -
BH100L_RS10830 2113621..2114445 - 825 WP_000754733.1 SDR family oxidoreductase -
BH100L_RS10835 2114528..2114782 - 255 WP_000767822.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
BH100L_RS10840 2114779..2115030 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
BH100L_RS10845 2115313..2115363 + 51 WP_001364200.1 his operon leader peptide -
BH100L_RS10855 2115509..2116408 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
BH100L_RS10860 2116414..2117718 + 1305 WP_001297912.1 histidinol dehydrogenase -
BH100L_RS10865 2117715..2118785 + 1071 WP_000108995.1 histidinol-phosphate transaminase -
BH100L_RS10870 2118785..2119852 + 1068 WP_000080122.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(5-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T91844 WP_000767822.1 NZ_CP025716:c2114782-2114528 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINEIIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T91844 NZ_CP025716:c2114782-2114528 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAAATTATCAAAGATACACGCAGAACACCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GCTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT91844 WP_001259255.1 NZ_CP025716:c2115030-2114779 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT91844 NZ_CP025716:c2115030-2114779 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1P977

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References