Detailed information of TA system TA091797

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yafN-yafO (relBE)/YafO-YafN
• Location: 3645242..3645936
• Replicon: chromosome
• Accession: NZ_CP025707
• Organism: Escherichia coli strain YDC107

Toxin (Protein)

• Gene name: yafO
• Uniprot ID: A0A0D8WHS4
• Locus tag: YDC107_RS17870
• Protein ID: WP_001521903.1
• Coordinates: 3645538..3645936 (+)
• Length: 133 a.a.

Antitoxin (Protein)

• Gene name: yafN
• Uniprot ID: S1QAE3
• Locus tag: YDC107_RS17865
• Protein ID: WP_000554758.1
• Coordinates: 3645242..3645535 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
YDC107_RS17840	3640357..3641106	+	750	WP_016233951.1	C40 family peptidase	-
YDC107_RS17845	3641163..3641516	-	354	WP_024166466.1	type II toxin-antitoxin system HicB family antitoxin	-
YDC107_RS17850	3641595..3643334	-	1740	WP_021523009.1	flagellar biosynthesis protein FlhA	-
YDC107_RS17855	3643294..3644064	+	771	WP_001301640.1	putative lateral flagellar export/assembly protein LafU	-
YDC107_RS17860	3644135..3645190	+	1056	WP_001226177.1	DNA polymerase IV	-
YDC107_RS17865	3645242..3645535	+	294	WP_000554758.1	type I toxin-antitoxin system antitoxin YafN	Antitoxin
YDC107_RS17870	3645538..3645936	+	399	WP_001521903.1	type II toxin-antitoxin system mRNA interferase toxin YafO	Toxin
YDC107_RS17875	3645946..3646398	+	453	WP_023144376.1	GNAT family N-acetyltransferase	-
YDC107_RS17880	3646643..3646897	+	255	WP_001521905.1	hypothetical protein	-
YDC107_RS17885	3646866..3647197	+	332	Protein_3482	hypothetical protein	-
YDC107_RS17890	3647254..3648711	-	1458	WP_001293009.1	cytosol nonspecific dipeptidase	-
YDC107_RS17895	3648972..3649430	+	459	WP_001291990.1	xanthine phosphoribosyltransferase	-
YDC107_RS17900	3649522..3650766	+	1245	WP_000189541.1	esterase FrsA	-
-	3650026..3650106	+	81	NuclAT_11	-	-
-	3650026..3650106	+	81	NuclAT_11	-	-
-	3650026..3650106	+	81	NuclAT_11	-	-
-	3650026..3650106	+	81	NuclAT_11	-	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase toxin YafO (27-130)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 133 a.a.
• Molecular weight: 15471.88 Da
• Isoelectric Point: 8.0949

>T91797 WP_001521903.1 NZ_CP025707:3645538-3645936 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Nucleotide

• Length: 399 bp

>T91797 NZ_CP025707:3645538-3645936 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11233.76 Da
• Isoelectric Point: 5.2170

>AT91797 WP_000554758.1 NZ_CP025707:3645242-3645535 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Nucleotide

• Length: 294 bp

>AT91797 NZ_CP025707:3645242-3645535 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• AlphaFold DB: A0A0D8WHS4

Antitoxin

• AlphaFold DB: S1QAE3