Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3645242..3645936 Replicon chromosome
Accession NZ_CP025707
Organism Escherichia coli strain YDC107

Toxin (Protein)


Gene name yafO Uniprot ID A0A0D8WHS4
Locus tag YDC107_RS17870 Protein ID WP_001521903.1
Coordinates 3645538..3645936 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag YDC107_RS17865 Protein ID WP_000554758.1
Coordinates 3645242..3645535 (+) Length 98 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YDC107_RS17840 3640357..3641106 + 750 WP_016233951.1 C40 family peptidase -
YDC107_RS17845 3641163..3641516 - 354 WP_024166466.1 type II toxin-antitoxin system HicB family antitoxin -
YDC107_RS17850 3641595..3643334 - 1740 WP_021523009.1 flagellar biosynthesis protein FlhA -
YDC107_RS17855 3643294..3644064 + 771 WP_001301640.1 putative lateral flagellar export/assembly protein LafU -
YDC107_RS17860 3644135..3645190 + 1056 WP_001226177.1 DNA polymerase IV -
YDC107_RS17865 3645242..3645535 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
YDC107_RS17870 3645538..3645936 + 399 WP_001521903.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
YDC107_RS17875 3645946..3646398 + 453 WP_023144376.1 GNAT family N-acetyltransferase -
YDC107_RS17880 3646643..3646897 + 255 WP_001521905.1 hypothetical protein -
YDC107_RS17885 3646866..3647197 + 332 Protein_3482 hypothetical protein -
YDC107_RS17890 3647254..3648711 - 1458 WP_001293009.1 cytosol nonspecific dipeptidase -
YDC107_RS17895 3648972..3649430 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -
YDC107_RS17900 3649522..3650766 + 1245 WP_000189541.1 esterase FrsA -
- 3650026..3650106 + 81 NuclAT_11 - -
- 3650026..3650106 + 81 NuclAT_11 - -
- 3650026..3650106 + 81 NuclAT_11 - -
- 3650026..3650106 + 81 NuclAT_11 - -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-130)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 133 a.a.        Molecular weight: 15471.88 Da        Isoelectric Point: 8.0949

>T91797 WP_001521903.1 NZ_CP025707:3645538-3645936 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGILPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T91797 NZ_CP025707:3645538-3645936 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTATTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCGTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGTGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAGCCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGAAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT91797 WP_000554758.1 NZ_CP025707:3645242-3645535 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT91797 NZ_CP025707:3645242-3645535 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCGAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A0D8WHS4


Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References