Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 543145..543647 Replicon chromosome
Accession NZ_CP025707
Organism Escherichia coli strain YDC107

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag YDC107_RS02920 Protein ID WP_000767829.1
Coordinates 543145..543399 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag YDC107_RS02925 Protein ID WP_001259255.1
Coordinates 543396..543647 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
YDC107_RS02890 538160..538387 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
YDC107_RS02895 538401..539459 - 1059 WP_000492324.1 transport protein YeeE -
YDC107_RS02900 539638..540996 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
YDC107_RS02905 540986..541048 - 63 WP_010723108.1 membrane protein YoeI -
YDC107_RS02910 541263..542192 - 930 WP_023154044.1 LysR family transcriptional regulator -
YDC107_RS02915 542238..543062 - 825 WP_000754758.1 SDR family oxidoreductase -
YDC107_RS02920 543145..543399 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
YDC107_RS02925 543396..543647 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
YDC107_RS02930 543930..543980 + 51 WP_001364200.1 his operon leader peptide -
YDC107_RS02935 544126..545025 + 900 WP_021523143.1 ATP phosphoribosyltransferase -
YDC107_RS02940 545031..546335 + 1305 WP_021523144.1 histidinol dehydrogenase -
YDC107_RS02945 546332..547402 + 1071 WP_000108937.1 histidinol-phosphate transaminase -
YDC107_RS02950 547402..548469 + 1068 WP_000080136.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T91784 WP_000767829.1 NZ_CP025707:c543399-543145 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T91784 NZ_CP025707:c543399-543145 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT91784 WP_001259255.1 NZ_CP025707:c543647-543396 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT91784 NZ_CP025707:c543647-543396 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACAGAAAAGGAC
ATTATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References