Detailed information of TA system TA091763

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4418103..4418705
• Replicon: chromosome
• Accession: NZ_CP025703
• Organism: Escherichia coli strain BH100N substr. MG2017

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: BH100N_RS22505
• Protein ID: WP_000897305.1
• Coordinates: 4418103..4418414 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: BH100N_RS22510
• Protein ID: WP_000356397.1
• Coordinates: 4418415..4418705 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
BH100N_RS22480	4414017..4414616	+	600	WP_001298594.1	glucose-1-phosphatase	-
BH100N_RS22485	4414610..4415482	+	873	WP_000920762.1	virulence factor BrkB family protein	-
BH100N_RS22490	4415479..4415916	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
BH100N_RS22495	4415961..4416902	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
BH100N_RS22500	4416966..4417874	-	909	WP_001298596.1	alpha/beta hydrolase	-
BH100N_RS22505	4418103..4418414	+	312	WP_000897305.1	hypothetical protein	Toxin
BH100N_RS22510	4418415..4418705	+	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
BH100N_RS22520	4419310..4419528	+	219	WP_001298592.1	ribbon-helix-helix domain-containing protein	-
BH100N_RS22525	4419743..4420672	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
BH100N_RS22530	4420669..4421304	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
BH100N_RS22535	4421301..4422203	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12172.16 Da
• Isoelectric Point: 9.7791

>T91763 WP_000897305.1 NZ_CP025703:4418103-4418414 [Escherichia coli]
ILFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T91763 NZ_CP025703:4418103-4418414 [Escherichia coli]
ATCCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT91763 WP_000356397.1 NZ_CP025703:4418415-4418705 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT91763 NZ_CP025703:4418415-4418705 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACAGCCTTC
GCGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGATC
TTATTTCTGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACCGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAAGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7