Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 1619410..1619972 Replicon chromosome
Accession NZ_CP025703
Organism Escherichia coli strain BH100N substr. MG2017

Toxin (Protein)


Gene name higB Uniprot ID S1Q1V8
Locus tag BH100N_RS08365 Protein ID WP_000605675.1
Coordinates 1619694..1619972 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1PAQ1
Locus tag BH100N_RS08360 Protein ID WP_000781370.1
Coordinates 1619410..1619694 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
BH100N_RS08345 1614591..1617638 + 3048 WP_011478186.1 formate dehydrogenase-N subunit alpha -
BH100N_RS08350 1617651..1618535 + 885 WP_001240581.1 formate dehydrogenase N subunit beta -
BH100N_RS08355 1618528..1619181 + 654 WP_000045647.1 formate dehydrogenase-N subunit gamma -
BH100N_RS08360 1619410..1619694 - 285 WP_000781370.1 HigA family addiction module antidote protein Antitoxin
BH100N_RS08365 1619694..1619972 - 279 WP_000605675.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
BH100N_RS08370 1620158..1621168 - 1011 WP_000642380.1 alcohol dehydrogenase AdhP -
BH100N_RS08375 1621302..1622999 - 1698 WP_000433462.1 malate dehydrogenase -
BH100N_RS08380 1623156..1623293 - 138 WP_099989695.1 stationary-phase-induced ribosome-associated protein -
BH100N_RS08385 1623395..1623610 - 216 WP_000495766.1 biofilm-dependent modulation protein -
BH100N_RS08395 1623955..1624386 + 432 WP_000152305.1 peroxiredoxin OsmC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(4-92)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-88)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10537.16 Da        Isoelectric Point: 7.3206

>T91745 WP_000605675.1 NZ_CP025703:c1619972-1619694 [Escherichia coli]
MIMNFRHKGLRDLFLLGKTSGVIPTQVKRLRHRLAVIDAACCLADIDMPGYRLHPLSGDRDGIWAISVSGNWRITFEFVN
GDAYILDYEDYH

Download         Length: 279 bp

>T91745 NZ_CP025703:c1619972-1619694 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10401.08 Da        Isoelectric Point: 8.8567

>AT91745 WP_000781370.1 NZ_CP025703:c1619694-1619410 [Escherichia coli]
MKMANHPRPGDIIQESLDELNVSLREFARAMEIAPSTASRLLTGKAALTPEMAIKLSVVIGSSPQMWLNLQNAWSLAEAE
KTVDVSRLRRLVTQ

Download         Length: 285 bp

>AT91745 NZ_CP025703:c1619694-1619410 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCAGAA
AAAACAGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1Q1V8

Antitoxin

Source ID Structure
PDB 2ICT
PDB 2ICP
AlphaFold DB A0A829CUG6

References