Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3843678..3844275 Replicon chromosome
Accession NZ_CP025684
Organism Klebsiella pneumoniae strain L5-2

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag C0067_RS19775 Protein ID WP_004142563.1
Coordinates 3843958..3844275 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag C0067_RS19770 Protein ID WP_004142561.1
Coordinates 3843678..3843965 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0067_RS19740 3839759..3840007 + 249 WP_002893037.1 DUF1158 domain-containing protein -
C0067_RS19745 3840025..3840366 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
C0067_RS19750 3840397..3841512 - 1116 WP_020316605.1 MBL fold metallo-hydrolase -
C0067_RS19755 3841691..3842275 + 585 WP_002893026.1 TetR/AcrR family transcriptional regulator -
C0067_RS19760 3842272..3842640 + 369 WP_040171587.1 MmcQ/YjbR family DNA-binding protein -
C0067_RS19765 3842760..3843413 + 654 WP_004178896.1 oxygen-insensitive NAD(P)H nitroreductase -
C0067_RS19770 3843678..3843965 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
C0067_RS19775 3843958..3844275 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C0067_RS19780 3844460..3845503 - 1044 WP_021464394.1 DUF2157 domain-containing protein -
C0067_RS19790 3846169..3847035 - 867 WP_004151823.1 helix-turn-helix transcriptional regulator -
C0067_RS19795 3847144..3848571 + 1428 WP_009308097.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T91728 WP_004142563.1 NZ_CP025684:c3844275-3843958 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T91728 NZ_CP025684:c3844275-3843958 [Klebsiella pneumoniae]
ATGTTCAGATTGGTTGTGCATGTGGACGTTAAAAAGGAATTGCAGGCGCTTCCGCCCATTGTCCAGGCGAAGATGATCCG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGACAGTAAACCGCTGGGGCAGGGGCTATTTGAAA
TTCGTGCGTTAGGTTCGGTACAGGGGCGAGGGATCTACGTTTTTCAGCAAGGCAAGACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAACCCCGTCATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT91728 WP_004142561.1 NZ_CP025684:c3843965-3843678 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT91728 NZ_CP025684:c3843965-3843678 [Klebsiella pneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTGGCCAGCCTGAGGTTCAGGCTGCATTCGATGC
GCAAGAACGTCAACAGCGATTACGTACTATGCTGGCGCAATGGCGCGAGCAGGCGGGGCTAACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCGACAGTCTCCAGAATGGAAGCCAATATCACCCGCGCCAGTCTGGATACCTTAACGCGA
TACGCGTTAGCCTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References