Detailed information of TA system TA091705

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 252379..252989
• Replicon: chromosome
• Accession: NZ_CP025682
• Organism: Azoarcus pumilus strain SY39

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: C0099_RS01250
• Protein ID: WP_102245754.1
• Coordinates: 252708..252989 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: graA
• Uniprot ID: G6YV52
• Locus tag: C0099_RS01245
• Protein ID: WP_008174257.1
• Coordinates: 252379..252693 (-)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0099_RS01230	248567..250387	+	1821	WP_102245751.1	glutamine--fructose-6-phosphate transaminase (isomerizing)	-
C0099_RS01235	250687..251241	-	555	WP_102245752.1	hypothetical protein	-
C0099_RS01240	251450..252202	-	753	WP_102245753.1	hypothetical protein	-
C0099_RS01245	252379..252693	-	315	WP_008174257.1	HigA family addiction module antidote protein	Antitoxin
C0099_RS01250	252708..252989	-	282	WP_102245754.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C0099_RS01255	253267..254766	+	1500	WP_102248334.1	IS21 family transposase	-
C0099_RS01260	254756..255568	+	813	WP_102245755.1	IS21-like element helper ATPase IstB	-
C0099_RS01265	255651..255867	+	217	Protein_254	GNAT family N-acetyltransferase	-
C0099_RS01270	256333..257688	+	1356	WP_199797638.1	acetamidase/formamidase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (28-74)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10691.12 Da
• Isoelectric Point: 7.8770

>T91705 WP_102245754.1 NZ_CP025682:c252989-252708 [Azoarcus pumilus]
MIKSFADKRTQELYAKGKSKKFPADVAPRAARKLEYVDLAVQLEDLKVPPGNRLHPLSGNRQGQHAISINDQWRICFRFE
DGDAYEVEVCDYH

Nucleotide

• Length: 282 bp

>T91705 NZ_CP025682:c252989-252708 [Azoarcus pumilus]
ATGATCAAATCCTTCGCTGACAAACGAACCCAAGAACTCTACGCCAAGGGCAAGTCAAAGAAATTTCCTGCGGATGTTGC
ACCGAGAGCCGCTAGAAAACTTGAATACGTGGATCTGGCCGTACAGCTTGAGGATTTGAAAGTGCCGCCCGGCAATCGCC
TTCACCCACTGTCGGGAAACAGGCAGGGGCAGCACGCAATTTCAATCAATGATCAGTGGCGTATCTGTTTTCGTTTTGAA
GATGGCGATGCGTATGAAGTCGAAGTGTGTGACTACCACTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 11644.33 Da
• Isoelectric Point: 7.2052

>AT91705 WP_008174257.1 NZ_CP025682:c252693-252379 [Azoarcus pumilus]
MAILNTAGMQRKPTHPGEMLREDFLPDYGLTVSGLAESLGVSRQSVNELLRERRAVSPEMALRLARLFGNSPEFWLNAQR
SVDLWTAAQSVKEEVDRIKPLHAA

Nucleotide

• Length: 315 bp

>AT91705 NZ_CP025682:c252693-252379 [Azoarcus pumilus]
ATGGCTATTCTTAATACTGCTGGCATGCAGCGCAAACCGACTCATCCTGGCGAAATGCTGAGGGAAGACTTTCTTCCGGA
CTATGGTCTGACGGTTTCGGGGTTGGCCGAATCTTTGGGTGTCTCTCGTCAGTCAGTGAATGAACTATTGCGGGAACGCC
GTGCTGTCAGCCCTGAAATGGCGCTCAGGCTTGCGCGTTTGTTTGGTAACTCGCCAGAATTCTGGCTGAACGCACAGCGG
TCTGTTGATCTTTGGACTGCTGCGCAGTCGGTCAAGGAAGAAGTGGATCGAATCAAGCCGCTACATGCTGCATAA

Structures

Antitoxin

• AlphaFold DB: G6YV52