Detailed information of TA system TA091704

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 237321..237930
• Replicon: chromosome
• Accession: NZ_CP025682
• Organism: Azoarcus pumilus strain SY39

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: C0099_RS01180
• Protein ID: WP_102245741.1
• Coordinates: 237619..237930 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: C0099_RS01175
• Protein ID: WP_199797667.1
• Coordinates: 237321..237539 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0099_RS01155	232773..234392	+	1620	WP_102245736.1	urea ABC transporter permease subunit UrtB	-
C0099_RS01160	234548..235756	+	1209	WP_102245737.1	urea ABC transporter permease subunit UrtC	-
C0099_RS01165	235758..236597	+	840	WP_102245738.1	urea ABC transporter ATP-binding protein UrtD	-
C0099_RS01170	236600..237295	+	696	WP_102245739.1	urea ABC transporter ATP-binding subunit UrtE	-
C0099_RS01175	237321..237539	-	219	WP_199797667.1	helix-turn-helix domain-containing protein	Antitoxin
C0099_RS01180	237619..237930	-	312	WP_102245741.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
C0099_RS01185	238039..238626	-	588	WP_123785202.1	hypothetical protein	-
C0099_RS01190	238728..238964	-	237	WP_102245743.1	antitoxin MazE family protein	-
C0099_RS01195	239076..239927	-	852	WP_102245744.1	DNA ligase	-
C0099_RS01200	240119..242272	-	2154	WP_102245745.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (44-99)

Antitoxin

• Cro/C1-type helix-turn-helix domain (15-50)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 11468.07 Da
• Isoelectric Point: 9.8618

>T91704 WP_102245741.1 NZ_CP025682:c237930-237619 [Azoarcus pumilus]
MLTVVETPLFQRQWPAYWTEEERGAFAAYIAEHPSAGDVVPESGGIRKVRWSRAGSGKSGGVRVIYFTRTAQGEIVLLTL
YAKARTDNISGAKLKEIRRALEE

Nucleotide

• Length: 312 bp

>T91704 NZ_CP025682:c237930-237619 [Azoarcus pumilus]
ATGCTGACAGTCGTCGAGACGCCTCTCTTCCAACGCCAGTGGCCGGCATACTGGACTGAAGAGGAGCGAGGCGCCTTCGC
CGCCTATATCGCGGAGCACCCAAGCGCCGGGGATGTCGTGCCGGAATCCGGCGGTATCCGGAAGGTGCGCTGGAGCCGCG
CCGGATCTGGCAAGTCAGGCGGCGTGCGCGTGATCTATTTCACCCGTACGGCTCAGGGCGAGATCGTATTGCTCACCCTC
TACGCGAAGGCCAGAACCGACAACATCAGTGGCGCAAAGCTGAAGGAGATCCGCCGTGCCCTCGAAGAATAA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 7471.70 Da
• Isoelectric Point: 12.1412

>AT91704 WP_199797667.1 NZ_CP025682:c237539-237321 [Azoarcus pumilus]
MKAGQLQVVTSPVIEARKKTGLSQSQFAALLGVSVRTLQGWEQGRKQPSGAARTLLAIASTNPQAVLAVAGK

Nucleotide

• Length: 219 bp

>AT91704 NZ_CP025682:c237539-237321 [Azoarcus pumilus]
ATGAAGGCTGGCCAACTGCAGGTTGTCACTTCTCCGGTCATCGAGGCGCGCAAAAAGACCGGGCTGTCGCAGTCTCAGTT
CGCGGCGCTGCTGGGGGTTTCGGTGCGCACCCTGCAGGGCTGGGAGCAGGGCCGCAAGCAGCCCAGTGGTGCGGCCCGGA
CCCTGCTTGCGATTGCCAGCACCAACCCGCAGGCGGTGCTTGCGGTAGCTGGAAAGTAG