Detailed information of TA system TA091680

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 21629..21882
• Replicon: plasmid pEA-1
• Accession: NZ_CP025677
• Organism: Escherichia albertii strain ChinaSP140150

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: G9G1E3
• Locus tag: C0R48_RS25340
• Protein ID: WP_001312851.1
• Coordinates: 21733..21882 (+)
• Length: 50 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	21629..21688 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
C0R48_RS25310	18356..19102	+	747	WP_000205770.1	conjugal transfer pilus acetylase TraX	-
C0R48_RS25315	19157..19717	+	561	WP_000139328.1	fertility inhibition protein FinO	-
C0R48_RS25320	19849..20049	+	201	WP_015059022.1	hypothetical protein	-
C0R48_RS25325	20435..21034	+	600	WP_032083981.1	hypothetical protein	-
C0R48_RS25330	21198..21428	+	231	WP_001736714.1	hypothetical protein	-
-	21629..21688	-	60	NuclAT_1	-	Antitoxin
-	21629..21688	-	60	NuclAT_1	-	Antitoxin
-	21629..21688	-	60	NuclAT_1	-	Antitoxin
-	21629..21688	-	60	NuclAT_1	-	Antitoxin
C0R48_RS25340	21733..21882	+	150	WP_001312851.1	type I toxin-antitoxin system Hok family toxin	Toxin
C0R48_RS25345	22166..22414	+	249	WP_000083837.1	replication regulatory protein RepA	-
C0R48_RS25355	22659..22733	+	75	WP_001375168.1	RepA leader peptide Tap	-
C0R48_RS25360	22726..23583	+	858	WP_032495102.1	incFII family plasmid replication initiator RepA	-
C0R48_RS25370	24522..25175	+	654	WP_000616807.1	CPBP family intramembrane metalloprotease	-
C0R48_RS27170	25268..25525	+	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
C0R48_RS25375	25458..25859	+	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
C0R48_RS25385	26150..26854	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	floR / tet(A) / aph(6)-Id / aph(3'')-Ib / sul2 / aph(3')-IIa / oqxB / oqxA / blaCTX-M-55	-	1..129356	129356

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-45)

Sequences

Toxin

Protein

• Length: 50 a.a.
• Molecular weight: 5542.67 Da
• Isoelectric Point: 8.7678

>T91680 WP_001312851.1 NZ_CP025677:21733-21882 [Escherichia albertii]
MTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 150 bp

>T91680 NZ_CP025677:21733-21882 [Escherichia albertii]
ATGACGAAATATGCCCTTATCGGGTTGCTCGCCGTGTGTGCCACGGTATTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAACTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin

Nucleotide

• Length: 60 bp

>AT91680 NZ_CP025677:c21688-21629 [Escherichia albertii]
TAAGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A829C8K5

Antitoxin