Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 1306524..1307088 Replicon chromosome
Accession NZ_CP025582
Organism Lacticaseibacillus paracasei strain HD1.7

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag CWS34_RS07075 Protein ID WP_016363448.1
Coordinates 1306786..1307088 (+) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag CWS34_RS07070 Protein ID WP_003570051.1
Coordinates 1306524..1306793 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CWS34_RS07040 1301529..1302416 - 888 WP_003570041.1 ABC transporter ATP-binding protein -
CWS34_RS07045 1302613..1303839 - 1227 WP_003570043.1 hypothetical protein -
CWS34_RS07050 1304037..1304441 + 405 WP_003570045.1 MerR family transcriptional regulator -
CWS34_RS07055 1304434..1304760 + 327 WP_003584272.1 YrdB family protein -
CWS34_RS07060 1304823..1305452 + 630 WP_101512010.1 DUF2399 domain-containing protein -
CWS34_RS07065 1305497..1306150 + 654 WP_003570049.1 GNAT family N-acetyltransferase -
CWS34_RS07070 1306524..1306793 + 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
CWS34_RS07075 1306786..1307088 + 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
CWS34_RS15990 1307427..1307570 + 144 WP_162259784.1 hypothetical protein -
CWS34_RS07080 1307688..1308380 + 693 WP_003570055.1 hypothetical protein -
CWS34_RS07085 1308616..1310793 + 2178 WP_003570057.1 RNA-binding transcriptional accessory protein -
CWS34_RS07090 1311218..1311775 - 558 WP_003584278.1 helix-turn-helix domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T90624 WP_016363448.1 NZ_CP025582:1306786-1307088 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T90624 NZ_CP025582:1306786-1307088 [Lacticaseibacillus paracasei]
ATGTATAGTCTGGTTCCGACGCCTACATTTAAGCGCGATCTAAAACGACTCTCCAAGAAGCATTGGCCGATGGACGAACT
AAAGACGGCTGTTAATCTCCTAGCCGCTGGTACAAATGCTGAACTATTAAGCAAAAAGTATGCAGATCATGCCTTGTCCT
CAAGCAGCGAGTGGAAAGGATATCGTGAACTATATGTTGACGGCCCTCGTGGCGACTGGTTGCTAATCTATAAAATTGAG
CAGCAAGATCTTATTTTAACCCTAGTTAGAACCGGATCTCATCATAACCTTCTGGTTAAATAG

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT90624 WP_003570051.1 NZ_CP025582:1306524-1306793 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT90624 NZ_CP025582:1306524-1306793 [Lacticaseibacillus paracasei]
ATGGCAGCCACAAAGAAGGAAACTCGCTTGAATATTCGTGTTGATCCTGAATTAAAAAGTGCTGCTCAAATCGTAGCAAA
TGATATGGGCATCGACTTGACCGCAGCTGTTACGATGTTCATGACGAAAATGGTGAAAGATCACGCCCTCCCGTTTACCC
CAACAAGTCTACCAGTTGAAACCTTACAGGCGCTGAAAGAAGCAAAGCACCCAGAGCTGCTCAAAAAATACAGCACGCCT
GATGACATGTGGAGAGACTTGAATGTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References