Detailed information of TA system TA090621

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 103573..104300
• Replicon: plasmid p08EU827_1
• Accession: NZ_CP025577
• Organism: Klebsiella pneumoniae strain 08EU827

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5Q9LNL2
• Locus tag: CY656_RS26570
• Protein ID: WP_004118600.1
• Coordinates: 103989..104300 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: V0AHC4
• Locus tag: CY656_RS26565
• Protein ID: WP_000990392.1
• Coordinates: 103573..103992 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY656_RS26530	99260..99622	-	363	WP_020324593.1	arsenite efflux transporter metallochaperone ArsD	-
CY656_RS26535	99670..100023	-	354	WP_001114073.1	As(III)-sensing metalloregulatory transcriptional repressor ArsR	-
CY656_RS28875	100339..100932	+	594	WP_044348674.1	hypothetical protein	-
CY656_RS26550	101356..102015	+	660	WP_000078540.1	hypothetical protein	-
CY656_RS26555	102012..102341	+	330	WP_004118613.1	hypothetical protein	-
CY656_RS26560	102334..103536	+	1203	WP_003031541.1	hypothetical protein	-
CY656_RS26565	103573..103992	-	420	WP_000990392.1	helix-turn-helix domain-containing protein	Antitoxin
CY656_RS26570	103989..104300	-	312	WP_004118600.1	type II toxin-antitoxin system HigB family toxin	Toxin
CY656_RS26575	104511..104891	+	381	Protein_106	gluconate permease	-
CY656_RS26580	104941..105636	-	696	WP_004118595.1	lactate utilization protein C	-
CY656_RS26585	105629..107056	-	1428	WP_000023895.1	iron-sulfur cluster-binding protein	-
CY656_RS26590	107067..107786	-	720	WP_001102107.1	(Fe-S)-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..215872	215872

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12386.14 Da
• Isoelectric Point: 10.0935

>T90621 WP_004118600.1 NZ_CP025577:c104300-103989 [Klebsiella pneumoniae]
MHIVSRAPFDTATRQFPNQAAALDDVYRTLKRENYTSPDEMKKRFASLDRMKYREKWWVIDVGGGNLRVMFFADFERGKI
FIKHITTHAEYDKLTDFYRRTKE

Nucleotide

• Length: 312 bp

>T90621 NZ_CP025577:c104300-103989 [Klebsiella pneumoniae]
ATGCACATTGTATCGAGAGCACCTTTCGATACAGCCACCAGGCAATTCCCCAATCAGGCAGCGGCACTAGACGACGTATA
CCGGACTTTGAAGAGAGAGAACTACACTTCACCGGACGAAATGAAAAAACGATTTGCCTCGCTGGACAGAATGAAATATC
GGGAGAAGTGGTGGGTGATCGATGTTGGCGGTGGGAATTTAAGGGTGATGTTTTTTGCTGATTTTGAACGTGGGAAAATC
TTTATAAAGCACATAACCACACACGCCGAATACGACAAGCTGACAGATTTTTACCGGAGAACTAAAGAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15534.71 Da
• Isoelectric Point: 4.4702

>AT90621 WP_000990392.1 NZ_CP025577:c103992-103573 [Klebsiella pneumoniae]
MMYTDAIQAANSLVSIVPLLGGNASRKDYEDALTLVEYLVEHEPDHPLVDMLVAKIAQYEDEAEEFAEFNDRIAALPSGV
ALLRVLMDQHKLTQSDFEEEIGKKSLVSRILNGTRSLTLDHMKALARRFNIPPSSFMDA

Nucleotide

• Length: 420 bp

>AT90621 NZ_CP025577:c103992-103573 [Klebsiella pneumoniae]
ATGATGTACACCGATGCTATCCAGGCGGCAAACAGCCTGGTTAGCATTGTCCCCCTCCTGGGGGGCAATGCCTCTCGCAA
GGACTATGAAGACGCGCTGACGCTGGTTGAATACCTGGTTGAACACGAGCCAGATCATCCGCTGGTGGACATGCTGGTTG
CGAAAATTGCCCAGTATGAAGATGAGGCGGAAGAGTTCGCAGAGTTCAACGACCGTATTGCAGCGTTACCAAGTGGCGTG
GCCCTGCTGCGTGTCCTGATGGACCAGCACAAACTGACTCAGAGTGATTTTGAGGAAGAGATTGGTAAGAAATCGCTGGT
GAGCCGCATCCTTAACGGGACTCGCTCACTAACACTAGATCACATGAAAGCACTTGCGCGGCGCTTCAACATTCCGCCCA
GCTCATTCATGGACGCGTAA

Structures

Toxin

• AlphaFold DB: A0A5Q9LNL2

Antitoxin

• AlphaFold DB: V0AHC4