Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type I Classification (family/domain) hok-sok/-
Location 83771..84040 Replicon plasmid pE1246_1
Accession NZ_CP025574
Organism Escherichia coli strain E-1246

Toxin (Protein)


Gene name hok Uniprot ID P11895
Locus tag CY655_RS27440 Protein ID WP_001312861.1
Coordinates 83882..84040 (+) Length 53 a.a.

Antitoxin (RNA)


Gene name sok
Locus tag -
Coordinates 83771..83836 (+)

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CY655_RS27415 79570..80091 + 522 WP_000290849.1 single-stranded DNA-binding protein -
CY655_RS27420 80147..80380 + 234 WP_000005970.1 DUF905 domain-containing protein -
CY655_RS27425 80439..82397 + 1959 Protein_98 ParB/RepB/Spo0J family partition protein -
CY655_RS27430 82452..82886 + 435 WP_000845949.1 conjugation system SOS inhibitor PsiB -
CY655_RS27435 82883..83602 + 720 WP_001403352.1 plasmid SOS inhibition protein A -
- 83614..83838 + 225 NuclAT_0 - -
- 83614..83838 + 225 NuclAT_0 - -
- 83614..83838 + 225 NuclAT_0 - -
- 83614..83838 + 225 NuclAT_0 - -
CY655_RS28000 83623..83802 - 180 WP_001309233.1 hypothetical protein -
- 83771..83836 + 66 - - Antitoxin
CY655_RS27440 83882..84040 + 159 WP_001312861.1 type I toxin-antitoxin system Hok family toxin Toxin
CY655_RS28005 84341..84572 - 232 Protein_103 hypothetical protein -
CY655_RS27455 84890..85177 + 288 WP_000107526.1 hypothetical protein -
CY655_RS27460 85296..86117 + 822 WP_001234469.1 DUF945 domain-containing protein -
CY655_RS27470 86412..87014 - 603 WP_023908348.1 transglycosylase SLT domain-containing protein -
CY655_RS27475 87336..87719 + 384 WP_001151538.1 relaxosome protein TraM -
CY655_RS27480 87906..88595 + 690 WP_032173224.1 conjugal transfer transcriptional regulator TraJ -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - agg3D / agg3C / agg3B / aap/aspU / pic 1..129195 129195


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(8-48)


Sequences


Toxin        


Download         Length: 53 a.a.        Molecular weight: 6082.32 Da        Isoelectric Point: 9.2584

>T90596 WP_001312861.1 NZ_CP025574:83882-84040 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Download         Length: 159 bp

>T90596 NZ_CP025574:83882-84040 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin


Download         Length: 66 bp

>AT90596 NZ_CP025574:83771-83836 [Escherichia coli]
AGAAGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCAC

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB P11895


Antitoxin

Download structure file

References