Detailed information of TA system TA090595

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 83770..84040
• Replicon: plasmid pE1246_1
• Accession: NZ_CP025574
• Organism: Escherichia coli strain E-1246

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CY655_RS27440
• Protein ID: WP_001312861.1
• Coordinates: 83882..84040 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	83770..83833 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY655_RS27415	79570..80091	+	522	WP_000290849.1	single-stranded DNA-binding protein	-
CY655_RS27420	80147..80380	+	234	WP_000005970.1	DUF905 domain-containing protein	-
CY655_RS27425	80439..82397	+	1959	Protein_98	ParB/RepB/Spo0J family partition protein	-
CY655_RS27430	82452..82886	+	435	WP_000845949.1	conjugation system SOS inhibitor PsiB	-
CY655_RS27435	82883..83602	+	720	WP_001403352.1	plasmid SOS inhibition protein A	-
-	83614..83838	+	225	NuclAT_0	-	-
-	83614..83838	+	225	NuclAT_0	-	-
-	83614..83838	+	225	NuclAT_0	-	-
-	83614..83838	+	225	NuclAT_0	-	-
CY655_RS28000	83623..83802	-	180	WP_001309233.1	hypothetical protein	-
-	83770..83833	-	64	-	-	Antitoxin
CY655_RS27440	83882..84040	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CY655_RS28005	84341..84572	-	232	Protein_103	hypothetical protein	-
CY655_RS27455	84890..85177	+	288	WP_000107526.1	hypothetical protein	-
CY655_RS27460	85296..86117	+	822	WP_001234469.1	DUF945 domain-containing protein	-
CY655_RS27470	86412..87014	-	603	WP_023908348.1	transglycosylase SLT domain-containing protein	-
CY655_RS27475	87336..87719	+	384	WP_001151538.1	relaxosome protein TraM	-
CY655_RS27480	87906..88595	+	690	WP_032173224.1	conjugal transfer transcriptional regulator TraJ	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	agg3D / agg3C / agg3B / aap/aspU / pic	1..129195	129195

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T90595 WP_001312861.1 NZ_CP025574:83882-84040 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T90595 NZ_CP025574:83882-84040 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT90595 NZ_CP025574:c83833-83770 [Escherichia coli]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin