Detailed information of TA system TA090586

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4777318..4777920
• Replicon: chromosome
• Accession: NZ_CP025573
• Organism: Escherichia coli strain E-1246

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: CY655_RS23650
• Protein ID: WP_000897305.1
• Coordinates: 4777318..4777629 (+)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: CY655_RS23655
• Protein ID: WP_000356395.1
• Coordinates: 4777630..4777920 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY655_RS23630	4772360..4772797	+	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
CY655_RS23635	4772842..4773783	+	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
CY655_RS23640	4774199..4776013	+	1815	WP_001550352.1	hypothetical protein	-
CY655_RS23645	4776181..4777089	-	909	WP_001550353.1	alpha/beta hydrolase	-
CY655_RS23650	4777318..4777629	+	312	WP_000897305.1	hypothetical protein	Toxin
CY655_RS23655	4777630..4777920	+	291	WP_000356395.1	helix-turn-helix domain-containing protein	Antitoxin
CY655_RS23660	4778005..4778226	+	222	WP_001550354.1	hypothetical protein	-
CY655_RS23665	4778278..4778556	+	279	WP_001315112.1	hypothetical protein	-
CY655_RS23675	4778975..4779193	+	219	WP_001295676.1	ribbon-helix-helix domain-containing protein	-
CY655_RS23680	4779412..4779654	+	243	WP_001309881.1	ribbon-helix-helix domain-containing protein	-
CY655_RS23685	4779836..4780765	-	930	WP_000027720.1	formate dehydrogenase accessory protein FdhE	-
CY655_RS23690	4780762..4781397	-	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
CY655_RS23695	4781394..4782296	-	903	WP_000331377.1	formate dehydrogenase O subunit beta	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T90586 WP_000897305.1 NZ_CP025573:4777318-4777629 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T90586 NZ_CP025573:4777318-4777629 [Escherichia coli]
ATGCTCTTCATCGAAACGGAAATTTTTACTGAAGATGTTCAAAAACTGCTGACCGACGATGAATTCAGTCGTTTTCAGTT
TTTCCTGGCCTTGAATCCTGACTATGGTGAGGTAATACCTGAAACTGGTGGGTTAAGAAAGGTTCGATGGGTGTCAGGAG
GAAAGGGGAAACGTGCTGGTGTCAGGGTTATTTACTTTCATCAGGTTAAACATTATGAGATCAGATTACTGCTTATTTAT
CGTAAGGGTATTAAAGATGATCTGTCTCCCCAGGAAAAAGCAATGCTCCGGTTGTTAAATACGAGGTGGTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10869.46 Da
• Isoelectric Point: 10.8907

>AT90586 WP_000356395.1 NZ_CP025573:4777630-4777920 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRHPSRTFEVDAMKIKEIRRASGLSQSKFAELISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT90586 NZ_CP025573:4777630-4777920 [Escherichia coli]
ATGGATAAGGCACTTTTTGAGCGGTTGACTCACAGCATGGCCCAGATGAATGAAATTATTGAAGGAACGCGACACCCTTC
ACGGACTTTTGAAGTTGATGCTATGAAAATCAAAGAAATACGCCGCGCTTCAGGGTTGTCACAATCTAAATTTGCCGAAC
TTATTTCCGTAAGCGTAGACACGTTGCGTAACTGGGAGCAGGGGCGACGTTCACCGACTGGGCCTGCAAAGGCTTTGCTG
CGAGCGATTGCCAATGATCCGCAACATGTGCTGCAGGCACTTAACCGTTAG

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7