Detailed information of TA system TA090584

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 3931060..3931859
• Replicon: chromosome
• Accession: NZ_CP025573
• Organism: Escherichia coli strain E-1246

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: F0JQM9
• Locus tag: CY655_RS19570
• Protein ID: WP_000347270.1
• Coordinates: 3931395..3931859 (+)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: A0A0V9H1L4
• Locus tag: CY655_RS19565
• Protein ID: WP_001551693.1
• Coordinates: 3931060..3931395 (+)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CY655_RS19550	3926845..3927615	-	771	WP_001551692.1	2-dehydro-3-deoxyglucarate aldolase	-
CY655_RS19555	3927631..3928965	-	1335	WP_000599651.1	galactarate/glucarate/glycerate transporter GarP	-
CY655_RS19560	3929340..3930911	+	1572	WP_001273738.1	galactarate dehydratase	-
CY655_RS19565	3931060..3931395	+	336	WP_001551693.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
CY655_RS19570	3931395..3931859	+	465	WP_000347270.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
CY655_RS19575	3931914..3932723	-	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
CY655_RS19580	3932972..3934252	+	1281	WP_001551694.1	tagatose-bisphosphate aldolase subunit KbaZ	-
CY655_RS19585	3934275..3934748	+	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
CY655_RS19590	3934759..3935538	+	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
CY655_RS19595	3935528..3936406	+	879	WP_001295548.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
CY655_RS19600	3936424..3936858	+	435	WP_000948837.1	PTS sugar transporter subunit IIA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	3922298..3931859	9561

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17806.22 Da
• Isoelectric Point: 9.6924

>T90584 WP_000347270.1 NZ_CP025573:3931395-3931859 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEEAH

Nucleotide

• Length: 465 bp

>T90584 NZ_CP025573:3931395-3931859 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTACAGCGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACGCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAGACAGAAGAAGCCCATTGA

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12347.87 Da
• Isoelectric Point: 4.6890

>AT90584 WP_001551693.1 NZ_CP025573:3931060-3931395 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGQDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFDTQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT90584 NZ_CP025573:3931060-3931395 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCGACACTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Toxin

• AlphaFold DB: A0A0V9NQ90

Antitoxin

• AlphaFold DB: A0A0V9H1L4