90545

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 957627..958219 Replicon chromosome
Accession NZ_CP025542
Organism Pseudomonas fluorescens strain 2P24

Toxin (Protein)


Gene name graT Uniprot ID -
Locus tag C0J56_RS04225 Protein ID WP_134922762.1
Coordinates 957941..958219 (-) Length 93 a.a.

Antitoxin (Protein)


Gene name graA Uniprot ID -
Locus tag C0J56_RS04220 Protein ID WP_116834128.1
Coordinates 957627..957932 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
C0J56_RS04200 952749..953648 + 900 WP_134922758.1 proline iminopeptidase-family hydrolase -
C0J56_RS04205 954147..955628 + 1482 WP_134922759.1 leucine-rich repeat domain-containing protein -
C0J56_RS04210 955729..956562 + 834 WP_134922760.1 oxidoreductase -
C0J56_RS04215 956585..957520 + 936 WP_134922761.1 AraC family transcriptional regulator -
C0J56_RS04220 957627..957932 - 306 WP_116834128.1 HigA family addiction module antidote protein Antitoxin
C0J56_RS04225 957941..958219 - 279 WP_134922762.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
C0J56_RS04235 958604..959113 - 510 WP_134922763.1 Lrp/AsnC family transcriptional regulator -
C0J56_RS04240 959314..960330 + 1017 WP_109753447.1 1-aminocyclopropane-1-carboxylate deaminase -
C0J56_RS04245 960459..960923 + 465 WP_134922764.1 transketolase -
C0J56_RS04250 960984..961406 + 423 WP_134922765.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(1-92)

Antitoxin

Cro/C1-type helix-turn-helix domain

(17-69)


Sequences

Toxin        


Download         Length: 93 a.a.        Molecular weight: 10435.82 Da        Isoelectric Point: 6.4455

>T90545 WP_134922762.1 NZ_CP025542:c958219-957941 [Pseudomonas fluorescens]
IIRSFRCADTQVLFETGKTRCWAAILSVAERKLGMLDAAVDLNDLRSPPGNRLEQLYGDREGQHSIRINGQYRICFVWGA
NGPENVEIVDYH

Download         Length: 279 bp

>T90545 NZ_CP025542:c958219-957941 [Pseudomonas fluorescens]
ATAATCAGAAGCTTCCGATGCGCCGACACCCAAGTGCTTTTTGAAACCGGCAAGACTCGGTGCTGGGCCGCCATTCTTTC
AGTGGCGGAACGAAAGCTGGGCATGCTCGATGCGGCGGTCGACCTCAACGATCTGCGCTCCCCGCCTGGAAACAGGCTGG
AACAGTTGTATGGCGACAGGGAAGGCCAACACAGCATCCGGATTAACGGTCAGTACCGCATCTGTTTTGTCTGGGGCGCC
AACGGCCCCGAAAATGTAGAAATCGTAGATTATCATTGA

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11131.89 Da        Isoelectric Point: 6.8700

>AT90545 WP_116834128.1 NZ_CP025542:c957932-957627 [Pseudomonas fluorescens]
MFKNGMRPVHPGEILKEEYLEPLGLTAAALARALHVSAPTVNDIVLMRRSVSADVALRLAAALDTSAQFWLNLQTAYDLR
TAEIAKGEQIHKEVERIPHCA

Download         Length: 306 bp

>AT90545 NZ_CP025542:c957932-957627 [Pseudomonas fluorescens]
ATGTTTAAGAATGGCATGAGGCCCGTACACCCTGGAGAAATTCTCAAGGAAGAGTATCTGGAACCGCTTGGCCTTACGGC
CGCTGCGCTCGCGAGGGCTTTGCACGTGTCTGCCCCCACCGTAAATGACATCGTATTGATGCGCCGTAGCGTCAGTGCGG
ATGTGGCCTTGCGCCTGGCAGCAGCACTGGACACGTCAGCGCAGTTTTGGCTGAACTTGCAGACTGCGTATGATTTACGT
ACAGCAGAGATTGCGAAAGGGGAACAGATCCATAAGGAAGTGGAAAGAATTCCTCACTGCGCCTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure

References