90506

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 3749852..3750546 Replicon chromosome
Accession NZ_CP025520
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name yafO Uniprot ID Q47157
Locus tag CDN75_RS19435 Protein ID WP_001263489.1
Coordinates 3750148..3750546 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID S1QAE3
Locus tag CDN75_RS19430 Protein ID WP_000554758.1
Coordinates 3749852..3750145 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDN75_RS19410 3745484..3745981 + 498 WP_000006255.1 REP-associated tyrosine transposase RayT -
CDN75_RS19415 3746205..3747917 - 1713 Protein_3529 flagellar biosynthesis protein FlhA -
CDN75_RS19420 3747889..3748674 + 786 WP_000207552.1 putative lateral flagellar export/assembly protein LafU -
CDN75_RS19425 3748745..3749800 + 1056 WP_001226164.1 DNA polymerase IV -
CDN75_RS19430 3749852..3750145 + 294 WP_000554758.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
CDN75_RS19435 3750148..3750546 + 399 WP_001263489.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
CDN75_RS19440 3750556..3751008 + 453 WP_001059892.1 GNAT family N-acetyltransferase -
CDN75_RS19445 3751326..3751532 + 207 Protein_3535 RtcB family protein -
CDN75_RS19450 3751528..3752049 + 522 Protein_3536 peptide chain release factor H -
CDN75_RS19455 3752106..3753563 - 1458 WP_001292994.1 cytosol nonspecific dipeptidase -
CDN75_RS19460 3753824..3754282 + 459 WP_001291990.1 xanthine phosphoribosyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15487.84 Da        Isoelectric Point: 7.4215

>T90506 WP_001263489.1 NZ_CP025520:3750148-3750546 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANENNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T90506 NZ_CP025520:3750148-3750546 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11233.76 Da        Isoelectric Point: 5.2170

>AT90506 WP_000554758.1 NZ_CP025520:3749852-3750145 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKPIKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT90506 NZ_CP025520:3749852-3750145 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A090J8B1

Antitoxin

Source ID Structure
AlphaFold DB S1QAE3

References