90489

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 947074..947576 Replicon chromosome
Accession NZ_CP025520
Organism Escherichia coli strain DH5alpha

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag CDN75_RS05255 Protein ID WP_000767829.1
Coordinates 947074..947328 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag CDN75_RS05260 Protein ID WP_001259255.1
Coordinates 947325..947576 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CDN75_RS05225 942089..942316 - 228 WP_046613325.1 sulfurtransferase TusA family protein -
CDN75_RS05230 942330..943388 - 1059 WP_000492339.1 transport protein YeeE -
CDN75_RS05235 943567..944925 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
CDN75_RS05240 944915..944977 - 63 WP_010723108.1 membrane protein YoeI -
CDN75_RS05245 945192..946121 - 930 WP_000803351.1 LysR family transcriptional regulator -
CDN75_RS05250 946167..946991 - 825 WP_000754737.1 SDR family oxidoreductase -
CDN75_RS05255 947074..947328 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
CDN75_RS05260 947325..947576 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
CDN75_RS05265 947859..947909 + 51 WP_001364200.1 his operon leader peptide -
CDN75_RS05275 948055..948954 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
CDN75_RS05280 948960..950264 + 1305 WP_000009594.1 histidinol dehydrogenase -
CDN75_RS05285 950261..951331 + 1071 WP_000108941.1 histidinol-phosphate transaminase -
CDN75_RS05290 951331..952398 + 1068 WP_000080105.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T90489 WP_000767829.1 NZ_CP025520:c947328-947074 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T90489 NZ_CP025520:c947328-947074 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT90489 WP_001259255.1 NZ_CP025520:c947576-947325 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT90489 NZ_CP025520:c947576-947325 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGCTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References