Detailed information of TA system TA090387

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 89179..89605
• Replicon: plasmid p44-2
• Accession: NZ_CP025463
• Organism: Klebsiella pneumoniae strain F44

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CYE02_RS30980
• Protein ID: WP_001312861.1
• Coordinates: 89179..89337 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	89381..89605 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYE02_RS30030	84234..84461	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
CYE02_RS30035	84555..85241	-	687	WP_015059009.1	PAS domain-containing protein	-
CYE02_RS30040	85432..85815	-	384	WP_000124981.1	relaxosome protein TraM	-
CYE02_RS30045	86092..86739	+	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
CYE02_RS30050	87036..87857	-	822	WP_001234445.1	DUF945 domain-containing protein	-
CYE02_RS30055	87968..88264	-	297	WP_001272251.1	hypothetical protein	-
CYE02_RS31695	88564..88941	+	378	Protein_120	hypothetical protein	-
CYE02_RS30980	89179..89337	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	89381..89605	-	225	NuclAT_0	-	Antitoxin
-	89381..89605	-	225	NuclAT_0	-	Antitoxin
-	89381..89605	-	225	NuclAT_0	-	Antitoxin
-	89381..89605	-	225	NuclAT_0	-	Antitoxin
CYE02_RS30080	89617..90336	-	720	WP_001276217.1	plasmid SOS inhibition protein A	-
CYE02_RS30085	90333..90767	-	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CYE02_RS30090	90836..92859	-	2024	Protein_124	ParB/RepB/Spo0J family partition protein	-
CYE02_RS30095	92920..93153	-	234	WP_000006003.1	DUF905 family protein	-
CYE02_RS30100	93211..93738	-	528	WP_000290834.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	blaKPC-2 / blaSHV-12 / rmtB / blaTEM-1B / blaCTX-M-65 / catA2	-	1..161580	161580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T90387 WP_001312861.1 NZ_CP025463:c89337-89179 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T90387 NZ_CP025463:c89337-89179 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT90387 NZ_CP025463:c89605-89381 [Klebsiella pneumoniae]
TCACACAGATTACCCGTAAACAGCCTGAATGAGCGGGTTATTTTCAGGAAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTACCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin