Detailed information of TA system TA090358

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 92021..92291
• Replicon: plasmid p69-2
• Accession: NZ_CP025458
• Organism: Klebsiella pneumoniae strain KP69

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: CYD98_RS30590
• Protein ID: WP_001312861.1
• Coordinates: 92133..92291 (+)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	92021..92084 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CYD98_RS29970	87732..88259	+	528	WP_000290834.1	single-stranded DNA-binding protein	-
CYD98_RS29975	88317..88550	+	234	WP_000006003.1	DUF905 family protein	-
CYD98_RS29980	88611..90634	+	2024	Protein_120	ParB/RepB/Spo0J family partition protein	-
CYD98_RS29985	90703..91137	+	435	WP_000845953.1	conjugation system SOS inhibitor PsiB	-
CYD98_RS29990	91134..91853	+	720	WP_001276217.1	plasmid SOS inhibition protein A	-
-	91865..92089	+	225	NuclAT_0	-	-
-	91865..92089	+	225	NuclAT_0	-	-
-	91865..92089	+	225	NuclAT_0	-	-
-	91865..92089	+	225	NuclAT_0	-	-
-	92021..92084	-	64	-	-	Antitoxin
CYD98_RS30590	92133..92291	+	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
CYD98_RS31240	92529..92906	-	378	Protein_124	hypothetical protein	-
CYD98_RS30015	93206..93502	+	297	WP_001272251.1	hypothetical protein	-
CYD98_RS30020	93613..94434	+	822	WP_001234445.1	DUF945 domain-containing protein	-
CYD98_RS30025	94731..95378	-	648	WP_015059008.1	transglycosylase SLT domain-containing protein	-
CYD98_RS30030	95655..96038	+	384	WP_000124981.1	relaxosome protein TraM	-
CYD98_RS30035	96229..96915	+	687	WP_015059009.1	PAS domain-containing protein	-
CYD98_RS30040	97009..97236	+	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	catA2 / blaCTX-M-65 / blaTEM-1B / rmtB / blaSHV-12 / blaKPC-2	-	1..128563	128563

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T90358 WP_001312861.1 NZ_CP025458:92133-92291 [Klebsiella pneumoniae]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T90358 NZ_CP025458:92133-92291 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 64 bp

>AT90358 NZ_CP025458:c92084-92021 [Klebsiella pneumoniae]
GACTAGACATAGGGATGCCTCGTGGTGGTTAATGAAAATTAACTTACTACGGGGCTATCTTCTT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin