Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/Txe-RelB
Location 234560..235095 Replicon plasmid p1
Accession NZ_CP025426
Organism Enterococcus faecium strain SC4

Toxin (Protein)


Gene name yoeB Uniprot ID R2PQC0
Locus tag CX663_RS15535 Protein ID WP_002314366.1
Coordinates 234823..235095 (+) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID Q3Y220
Locus tag CX663_RS15530 Protein ID WP_002297422.1
Coordinates 234560..234823 (+) Length 88 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CX663_RS15515 229988..232237 + 2250 WP_002314387.1 alpha-galactosidase -
CX663_RS15520 232240..233415 + 1176 WP_002336205.1 MFS transporter -
CX663_RS15525 233418..234326 - 909 Protein_252 IS982 family transposase -
CX663_RS15530 234560..234823 + 264 WP_002297422.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
CX663_RS15535 234823..235095 + 273 WP_002314366.1 Txe/YoeB family addiction module toxin Toxin
CX663_RS15540 235172..235501 + 330 WP_002292678.1 AbrB family transcriptional regulator -
CX663_RS15545 235491..235777 + 287 Protein_256 type II toxin-antitoxin system RelE/ParE family toxin -
CX663_RS15550 236063..236920 + 858 WP_002292680.1 AAA family ATPase -
CX663_RS15555 236921..237109 + 189 Protein_258 hypothetical protein -
CX663_RS15560 237168..237848 - 681 WP_002331383.1 IS6-like element IS1216 family transposase -
CX663_RS15565 237924..238567 - 644 Protein_260 transposase -
CX663_RS16810 238842..239384 + 543 WP_002298088.1 hypothetical protein -
CX663_RS15580 239540..239989 + 450 WP_010729386.1 TlpA family protein disulfide reductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid aph(2'')-Ia / aac(6')-aph(2'') - 1..253120 253120
- inside IScluster/Tn - - 223959..246658 22699


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(9-87)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-69)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10780.24 Da        Isoelectric Point: 9.9724

>T90323 WP_002314366.1 NZ_CP025426:234823-235095 [Enterococcus faecium]
MNNYSVMIKNSAKADLKKIKQSNLKPQFEKVIQTLKEDPYLPTQSFEKLKPTHEGRYSRRLNRQHRVVYKVDEDEHVVEI
YSARTHYESQ

Download         Length: 273 bp

>T90323 NZ_CP025426:234823-235095 [Enterococcus faecium]
ATGAATAACTATTCTGTGATGATTAAAAACTCGGCTAAAGCTGATTTAAAGAAAATCAAACAGTCTAATTTGAAACCTCA
ATTTGAGAAGGTTATTCAGACTTTAAAGGAAGATCCCTATCTCCCTACCCAATCATTTGAGAAGTTAAAACCTACACATG
AAGGTAGGTATTCTAGAAGATTGAATAGACAACACCGGGTTGTTTATAAGGTAGATGAAGATGAACACGTGGTTGAAATT
TATTCAGCAAGGACGCATTACGAGTCACAGTAA

Antitoxin


Download         Length: 88 a.a.        Molecular weight: 9959.22 Da        Isoelectric Point: 4.5370

>AT90323 WP_002297422.1 NZ_CP025426:234560-234823 [Enterococcus faecium]
MAKTVLNPSSARKNFYKLLKDVNENHTEIEIISERSENNAVLIGLDDWKAIKETLLLEQIGTLGVVRKREKDDSGFTNVD
EIDWDNL

Download         Length: 264 bp

>AT90323 NZ_CP025426:234560-234823 [Enterococcus faecium]
ATGGCAAAAACAGTCTTAAATCCTAGCTCAGCACGGAAAAATTTTTATAAGTTGTTGAAAGATGTAAATGAGAATCATAC
GGAAATTGAAATCATTAGTGAGCGCAGTGAAAATAATGCGGTGCTTATTGGTTTGGATGATTGGAAAGCAATCAAAGAAA
CGCTATTACTTGAGCAAATAGGCACTTTGGGTGTTGTTCGTAAGCGAGAAAAAGATGATAGTGGATTTACTAATGTTGAT
GAAATTGATTGGGATAACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829F704

Antitoxin

Source ID Structure
AlphaFold DB A0A829F006

References